Detailed information of TA system TA223124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3662417..3663201
• Replicon: chromosome
• Accession: NZ_CP086369
• Organism: Pectobacterium versatile strain A73-S18-O15

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LLE50_RS16575
• Protein ID: WP_116586074.1
• Coordinates: 3662707..3663201 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: LLE50_RS16570
• Protein ID: WP_125232377.1
• Coordinates: 3662417..3662710 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE50_RS16545 (LLE50_16535)	3657515..3658726	+	1212	WP_103861689.1	glycosyltransferase family 4 protein	-
LLE50_RS16550 (LLE50_16540)	3658728..3659840	+	1113	WP_039507741.1	glycosyltransferase family 4 protein	-
LLE50_RS16555 (LLE50_16545)	3659843..3660250	+	408	WP_130621810.1	hypothetical protein	-
LLE50_RS16560 (LLE50_16550)	3660289..3661683	+	1395	WP_130621811.1	DUF4832 domain-containing protein	-
LLE50_RS16565 (LLE50_16555)	3661710..3662210	+	501	WP_103861241.1	serine acetyltransferase	-
LLE50_RS16570 (LLE50_16560)	3662417..3662710	+	294	WP_125232377.1	DUF1778 domain-containing protein	Antitoxin
LLE50_RS16575 (LLE50_16565)	3662707..3663201	+	495	WP_116586074.1	GNAT family N-acetyltransferase	Toxin
LLE50_RS16580 (LLE50_16570)	3663263..3663433	+	171	Protein_3233	helix-turn-helix domain-containing protein	-
LLE50_RS16585 (LLE50_16575)	3663772..3664065	+	294	WP_130621812.1	DUF1778 domain-containing protein	-
LLE50_RS16590 (LLE50_16580)	3664062..3664556	+	495	WP_130621813.1	GNAT family N-acetyltransferase	-
LLE50_RS16595 (LLE50_16585)	3664982..3666712	+	1731	WP_130621814.1	AAA family ATPase	-
LLE50_RS16600 (LLE50_16590)	3666798..3667640	+	843	WP_130621815.1	DNA adenine methylase	-
LLE50_RS16605 (LLE50_16595)	3667840..3668145	-	306	WP_130621816.1	type II toxin-antitoxin system toxin CcdB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3648153..3672248	24095

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (76-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17571.33 Da
• Isoelectric Point: 7.9094

>T223124 WP_116586074.1 NZ_CP086369:3662707-3663201 [Pectobacterium versatile]
MISAPEPLHAEHVFSSFGCGVESMDNWLKQRAMKNQVTGASRTFVSCDGSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARNFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T223124 NZ_CP118536:c2761127-2761025 [Salmonella enterica subsp. enterica serovar Typhi]
GCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10958.58 Da
• Isoelectric Point: 10.1968

>AT223124 WP_125232377.1 NZ_CP086369:3662417-3662710 [Pectobacterium versatile]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEVLIDQRIIMADPDAYQEFLARLDQTPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT223124 NZ_CP118536:2761023-2761166 [Salmonella enterica subsp. enterica serovar Typhi]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG