Detailed information of TA system TA223115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2775160..2775794
• Replicon: chromosome
• Accession: NZ_CP086369
• Organism: Pectobacterium versatile strain A73-S18-O15

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LLE50_RS12555
• Protein ID: WP_103862315.1
• Coordinates: 2775160..2775435 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A221T3Z5
• Locus tag: LLE50_RS12560
• Protein ID: WP_010308714.1
• Coordinates: 2775432..2775794 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE50_RS12520	2770646..2770711	-	66	WP_228925434.1	GpE family phage tail protein	-
LLE50_RS12525 (LLE50_12520)	2770795..2771106	-	312	WP_015840863.1	phage tail assembly protein	-
LLE50_RS12530 (LLE50_12525)	2771122..2771637	-	516	WP_015840864.1	phage major tail tube protein	-
LLE50_RS12535 (LLE50_12530)	2771648..2772841	-	1194	WP_130622423.1	phage tail sheath protein	-
LLE50_RS12540 (LLE50_12535)	2772998..2774131	+	1134	WP_130622424.1	phage late control D family protein	-
LLE50_RS12545 (LLE50_12540)	2774182..2774424	+	243	WP_039490579.1	ogr/Delta-like zinc finger family protein	-
LLE50_RS12550 (LLE50_12545)	2774436..2774915	+	480	WP_130622425.1	hypothetical protein	-
LLE50_RS12555 (LLE50_12550)	2775160..2775435	+	276	WP_103862315.1	type II toxin-antitoxin system HicA family toxin	Toxin
LLE50_RS12560 (LLE50_12555)	2775432..2775794	+	363	WP_010308714.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LLE50_RS12565 (LLE50_12560)	2775838..2777030	-	1193	Protein_2454	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10006.60 Da
• Isoelectric Point: 11.1875

>T223115 WP_103862315.1 NZ_CP086369:2775160-2775435 [Pectobacterium versatile]
MQGKIASLRKKQRETLSHIFKTPVLSGVKWSSIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T223115 NZ_CP118532:1705712-1706014 [Vibrio harveyi]
ATGGCTGAAATAATCTGGACTGAGCCAGCGCTATCTGACCTCAATGATATCGCTGAATACATCGCACTTGAAAATATCGT
AGCTGCAAAACAATTGGTTCAAGCGATCTTTTCTAAAGTCGAGCGCTTAGAGGCTTTCCCTGAATCGGGTCGCATTCCGC
CTGAACTAGAACATCTAAGTTATCGTGAAGTTGTAGTTAATCCGTGCCGTATTTTCTATAAACAAGACGGCGACAAAGTA
TTTATTTTGTTTGTTATGCGTGCTGAGAGAGACTTGCGTAAGTTCCTATTGAGCAAGCAATAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13495.29 Da
• Isoelectric Point: 4.6225

>AT223115 WP_010308714.1 NZ_CP086369:2775432-2775794 [Pectobacterium versatile]
MTKALNTPNTMVVSGQPAIISYVPEIGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERHISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT223115 NZ_CP118532:1705467-1705724 [Vibrio harveyi]
ATGAAAGTAGAACTAGTTACATCACTCAAACGCCAAGCAACAAAGATCCTTGCCGATCTCCACGACACTAAAGAACCTGT
GTTAATTACCGAGCATGGTAAGCCATCGGCATATCTGGTTGATGTTGATGATTACGAGTTTATGCAAAATCGTTTAGCTA
TTCTTGAGGGGATTGCACGAGGCGAACGTGCATTAGCTGACGGTAAAGTGGTGAGTCACGATGAAGCCAAGGACAAAATG
TCAAAATGGCTGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A221T3Z5