Detailed information of TA system TA223111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2361710..2362320
• Replicon: chromosome
• Accession: NZ_CP086369
• Organism: Pectobacterium versatile strain A73-S18-O15

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LLE50_RS10680
• Protein ID: WP_130621219.1
• Coordinates: 2361928..2362320 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: C6DIE5
• Locus tag: LLE50_RS10675
• Protein ID: WP_010300679.1
• Coordinates: 2361710..2361931 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE50_RS10650 (LLE50_10645)	2356857..2357765	+	909	WP_130621216.1	LysR family transcriptional regulator	-
LLE50_RS10655 (LLE50_10650)	2357863..2358726	+	864	WP_130621217.1	YicC/YloC family endoribonuclease	-
LLE50_RS10660 (LLE50_10655)	2358832..2359719	-	888	WP_130621218.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
LLE50_RS10665 (LLE50_10660)	2359748..2360272	-	525	WP_103862785.1	hypothetical protein	-
LLE50_RS10670 (LLE50_10665)	2361338..2361661	+	324	WP_103862786.1	hypothetical protein	-
LLE50_RS10675 (LLE50_10670)	2361710..2361931	+	222	WP_010300679.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LLE50_RS10680 (LLE50_10675)	2361928..2362320	+	393	WP_130621219.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LLE50_RS10685 (LLE50_10680)	2362660..2363100	+	441	WP_205076542.1	class I SAM-dependent methyltransferase	-
LLE50_RS10690 (LLE50_10685)	2363135..2363446	+	312	WP_198673504.1	hypothetical protein	-
LLE50_RS10695 (LLE50_10690)	2363722..2364279	+	558	WP_130621220.1	hypothetical protein	-
LLE50_RS10700 (LLE50_10695)	2364322..2364867	-	546	WP_181919109.1	hypothetical protein	-
LLE50_RS10705 (LLE50_10700)	2364981..2365367	+	387	WP_181919110.1	hypothetical protein	-
LLE50_RS10710 (LLE50_10705)	2365410..2365772	+	363	WP_130621221.1	DUF596 domain-containing protein	-
LLE50_RS10715 (LLE50_10710)	2365839..2367020	-	1182	WP_107332124.1	AI-2E family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (3-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14693.63 Da
• Isoelectric Point: 6.6442

>T223111 WP_130621219.1 NZ_CP086369:2361928-2362320 [Pectobacterium versatile]
MNWVSAQEVIAFHDRILRHLPGVAGMPDPGRAEAIIYRVQNRTHYEGITDVFELAAIYWVAVARGHIFNDGNKRTAFFVT
MTFLYRNGVLIRDNDNTLENLTVEAATGAKNVDQLAQHLRNLADEISPTR

Nucleotide

• Length: 393 bp

>T223111 NZ_CP118530:1362231-1362533 [Vibrio harveyi]
ATGGCTGAAATAATCTGGACTGAGCCAGCGCTATCTGACCTCAATGATATCGCTGAATACATCGCACTTGAAAATATCGT
AGCTGCAAAACAATTGGTTCAAGCGATCTTTTCTAAAGTCGAGCGCTTAGAGGCTTTCCCTGAATCGGGTCGCATTCCGC
CTGAACTAGAACATCTAAGTTATCGTGAAGTTGTAGTTAATCCGTGCCGTATTTTCTATAAACAAGACGGCGACAAAGTA
TTTATTTTGTTTGTTATGCGTGCTGAGAGAGACTTGCGTAAGTTCCTATTGAGCAAGCAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8135.01 Da
• Isoelectric Point: 5.0359

>AT223111 WP_010300679.1 NZ_CP086369:2361710-2361931 [Pectobacterium versatile]
MRTYTSTQARANISEVLDAATHGEPVEITRRDGSSAVVISKAEFEAYQNAKLDAEFDLIMQRHGHTIEALTDR

Nucleotide

• Length: 222 bp

>AT223111 NZ_CP118530:1361986-1362243 [Vibrio harveyi]
ATGAAAGTAGAACTAGTTACATCACTCAAACGCCAAGCAACAAAGATCCTTGCCGATCTCCACGACACTAAAGAACCTGT
GTTAATTACCGAGCATGGTAAGCCATCGGCATATCTGGTTGATGTTGATGATTACGAGTTTATGCAAAATCGTTTAGCTA
TTCTTGAGGGGATTGCACGAGGCGAACGTGCATTAGCTGACGGTAAAGTGGTGAGTCACGATGAAGCCAAGGACAAAATG
TCAAAATGGCTGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A221TFG2