Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 2785961..2786481 Replicon chromosome
Accession NZ_CP086350
Organism Clavibacter michiganensis subsp. nebraskensis strain 419B

Toxin (Protein)


Gene name parE Uniprot ID M5BDX6
Locus tag LIX20_RS13190 Protein ID WP_015491236.1
Coordinates 2785961..2786251 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A399PXI2
Locus tag LIX20_RS13195 Protein ID WP_041465310.1
Coordinates 2786248..2786481 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIX20_RS13180 (LIX20_002632) 2783910..2785508 + 1599 WP_015491235.1 class I SAM-dependent methyltransferase -
LIX20_RS13185 (LIX20_002634) 2785667..2785921 + 255 Protein_2589 helix-turn-helix transcriptional regulator -
LIX20_RS13190 (LIX20_002635) 2785961..2786251 - 291 WP_015491236.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LIX20_RS13195 (LIX20_002636) 2786248..2786481 - 234 WP_041465310.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
LIX20_RS13200 (LIX20_002637) 2786593..2786793 - 201 WP_015491238.1 hypothetical protein -
LIX20_RS13205 (LIX20_002638) 2786809..2787120 - 312 WP_236685126.1 antitoxin VbhA family protein -
LIX20_RS13210 (LIX20_002639) 2787128..2788192 + 1065 WP_237580721.1 LD-carboxypeptidase -
LIX20_RS13215 (LIX20_002640) 2788369..2788728 + 360 WP_015491241.1 hypothetical protein -
LIX20_RS13220 (LIX20_002641) 2788925..2789404 + 480 WP_015491243.1 hypothetical protein -
LIX20_RS13225 (LIX20_002642) 2789551..2790588 + 1038 WP_248873449.1 DUF1266 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-92)

Antitoxin

Antitoxin ParD

(4-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11338.63 Da        Isoelectric Point: 6.4832

>T223105 WP_015491236.1 NZ_CP086350:c2786251-2785961 [Clavibacter michiganensis subsp. nebraskensis]
VKPHRLTPAATKDLSEIWDYTEERWDADQAETYVREIGAAIRRVAEDPTRGRDCGDIRPGYRRYSIESHTVYYVDRPETV
DVIRILHQRMDPSRHL

Download         Length: 291 bp

>T223105 NZ_CP118496:1596042-1596329 [Pseudoalteromonas rhizosphaerae]
ATGACTTATAAGCTCAAATTTTTACCCGCAGCGCAAAAAGAGTGGAACAAGCTTGCTCCTCCTATAAAAAGTCAGTTTAA
AAAGAAACTCATAGAGCGTCTAGAAAATCCTCATGTACCAGCATCGAAACTGAGGGGTTTTGATAGTGTATATAAAATAA
AATTGCGCACAGCAGGCTACAGGCTTGCCTATGAAGTGATTGATGATGAAGTTGTAGTATATGTACTCGCTATCGGCAAA
CGAGATAAAGATGCAGTGTATAAAAAGTTGTCATCGCGCATTAAATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8268.18 Da        Isoelectric Point: 4.4000

>AT223105 WP_041465310.1 NZ_CP086350:c2786481-2786248 [Clavibacter michiganensis subsp. nebraskensis]
MAQNTSISLDGHFTGFLAREVESGRYRSASEVVRAGLRLLEDQETQLEALRAALVAGEASGEAAPFDLDAFIADKRA

Download         Length: 234 bp

>AT223105 NZ_CP118496:1595801-1596052 [Pseudoalteromonas rhizosphaerae]
ATGAGACAAATATTAGCAGACTGTTCAGCAAGTATTTCAGAATTAAAGAAAAATCCGACAGCGTTATTAAATGAAGCTGA
TGGTTCAGCGATAGCTATTTTAAATCACAATAAACCAGCAGCTTATTTAGTACCTGCGCAAACGTATGAGTTCCTAATGG
AACTGATCGACGATATTGAATTAGCAAAAATTGTCGAAAGTCGCAGAGCAGACTTATCAGAGGCTGTTGAAGTTAATATT
GATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M5BDX6

Antitoxin

Source ID Structure
AlphaFold DB A0A399PXI2

References