Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 2785570..2786090 Replicon chromosome
Accession NZ_CP086346
Organism Clavibacter michiganensis subsp. nebraskensis strain 44

Toxin (Protein)


Gene name parE Uniprot ID M5BDX6
Locus tag LIX22_RS13195 Protein ID WP_015491236.1
Coordinates 2785570..2785860 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A399PXI2
Locus tag LIX22_RS13200 Protein ID WP_041465310.1
Coordinates 2785857..2786090 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIX22_RS13185 (LIX22_002631) 2783519..2785117 + 1599 WP_015491235.1 class I SAM-dependent methyltransferase -
LIX22_RS13190 (LIX22_002633) 2785276..2785530 + 255 Protein_2590 helix-turn-helix transcriptional regulator -
LIX22_RS13195 (LIX22_002634) 2785570..2785860 - 291 WP_015491236.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LIX22_RS13200 (LIX22_002635) 2785857..2786090 - 234 WP_041465310.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
LIX22_RS13205 (LIX22_002636) 2786202..2786402 - 201 WP_015491238.1 hypothetical protein -
LIX22_RS13210 (LIX22_002637) 2786418..2786738 - 321 WP_227077692.1 antitoxin VbhA family protein -
LIX22_RS13215 (LIX22_002638) 2786737..2787801 + 1065 WP_237580721.1 LD-carboxypeptidase -
LIX22_RS13220 (LIX22_002639) 2787978..2788337 + 360 WP_015491241.1 hypothetical protein -
LIX22_RS13225 (LIX22_002640) 2788534..2789013 + 480 WP_015491243.1 hypothetical protein -
LIX22_RS13230 (LIX22_002641) 2789160..2790197 + 1038 WP_248873449.1 DUF1266 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-92)

Antitoxin

Antitoxin ParD

(4-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11338.63 Da        Isoelectric Point: 6.4832

>T223102 WP_015491236.1 NZ_CP086346:c2785860-2785570 [Clavibacter michiganensis subsp. nebraskensis]
VKPHRLTPAATKDLSEIWDYTEERWDADQAETYVREIGAAIRRVAEDPTRGRDCGDIRPGYRRYSIESHTVYYVDRPETV
DVIRILHQRMDPSRHL

Download         Length: 291 bp

>T223102 NZ_CP118494:3067095-3067406 [Roseovarius tolerans]
ATGAAACATGAAATCAACGATTTCGATCCGGCTGAGTATCTCGACAACGATGAAATGATCGCCGGCTACCTATCGGATGC
GCTTGAAACGCAAGATCCCGCATTCATCGCTGATGCGATCGGTGTTGTTGCGCGAGCTAAAGGGATGAAGCGCGTCGCTG
AAGATGCAGGTGTTAGCCGCGAGAGCCTCTATCGTGCGCTGAGCGCCAACGGGAACCCTGAGTTCGGTACCGTGCTCAAG
GTATTGGCCTCGCTCAACATAAAGCTGATTGCGCAGCCTGATAGTGTCAAGCCGCATAACACGGCGGCGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8268.18 Da        Isoelectric Point: 4.4000

>AT223102 WP_041465310.1 NZ_CP086346:c2786090-2785857 [Clavibacter michiganensis subsp. nebraskensis]
MAQNTSISLDGHFTGFLAREVESGRYRSASEVVRAGLRLLEDQETQLEALRAALVAGEASGEAAPFDLDAFIADKRA

Download         Length: 234 bp

>AT223102 NZ_CP118494:3066803-3067105 [Roseovarius tolerans]
ATGTTCGTAATCAGGCAAACAGACATCTTTTCTTCCTGGCTGCGCAATTTGCGTGACCAGCGGGCGAAGCAAAAAATCAC
CAGTCGGTTGCAGCGACTGAAGTTCGGGCATTTTGGCGATGTTGAACCTGTAGGTCATGGCGTGAGTGAGGTGCGCATCC
ATGAGGGAAAAGGCTACCGCGTCTATTTTCGCCAGCAAGGGGATGAGATCATCTTTCTGCTGTGCGGAGGTGACAAGAAA
AGCCAACAGCGAGACATCACGAAGGCGCAGCGCATCTGGAAGGAACTTGGAAATGAAACATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M5BDX6

Antitoxin

Source ID Structure
AlphaFold DB A0A399PXI2

References