Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 2776614..2777134 Replicon chromosome
Accession NZ_CP086345
Organism Clavibacter michiganensis subsp. nebraskensis strain CNK-2

Toxin (Protein)


Gene name parE Uniprot ID M5BDX6
Locus tag LIV34_RS13120 Protein ID WP_015491236.1
Coordinates 2776614..2776904 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A399PXI2
Locus tag LIV34_RS13125 Protein ID WP_041465310.1
Coordinates 2776901..2777134 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIV34_RS13110 (LIV34_002619) 2774563..2776161 + 1599 WP_015491235.1 class I SAM-dependent methyltransferase -
LIV34_RS13115 (LIV34_002621) 2776320..2776574 + 255 Protein_2577 helix-turn-helix transcriptional regulator -
LIV34_RS13120 (LIV34_002622) 2776614..2776904 - 291 WP_015491236.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LIV34_RS13125 (LIV34_002623) 2776901..2777134 - 234 WP_041465310.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
LIV34_RS13130 (LIV34_002624) 2777246..2777446 - 201 WP_015491238.1 hypothetical protein -
LIV34_RS13135 (LIV34_002625) 2777462..2777773 - 312 WP_236685126.1 antitoxin VbhA family protein -
LIV34_RS13140 (LIV34_002626) 2777781..2778845 + 1065 WP_045929310.1 LD-carboxypeptidase -
LIV34_RS13145 (LIV34_002627) 2779022..2779381 + 360 WP_045929311.1 hypothetical protein -
LIV34_RS13150 (LIV34_002628) 2779542..2780057 + 516 WP_041465311.1 hypothetical protein -
LIV34_RS13155 (LIV34_002629) 2780204..2781241 + 1038 WP_045929312.1 DUF1266 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-92)

Antitoxin

Antitoxin ParD

(4-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11338.63 Da        Isoelectric Point: 6.4832

>T223101 WP_015491236.1 NZ_CP086345:c2776904-2776614 [Clavibacter michiganensis subsp. nebraskensis]
VKPHRLTPAATKDLSEIWDYTEERWDADQAETYVREIGAAIRRVAEDPTRGRDCGDIRPGYRRYSIESHTVYYVDRPETV
DVIRILHQRMDPSRHL

Download         Length: 291 bp

>T223101 NZ_CP118494:3066803-3067105 [Roseovarius tolerans]
ATGTTCGTAATCAGGCAAACAGACATCTTTTCTTCCTGGCTGCGCAATTTGCGTGACCAGCGGGCGAAGCAAAAAATCAC
CAGTCGGTTGCAGCGACTGAAGTTCGGGCATTTTGGCGATGTTGAACCTGTAGGTCATGGCGTGAGTGAGGTGCGCATCC
ATGAGGGAAAAGGCTACCGCGTCTATTTTCGCCAGCAAGGGGATGAGATCATCTTTCTGCTGTGCGGAGGTGACAAGAAA
AGCCAACAGCGAGACATCACGAAGGCGCAGCGCATCTGGAAGGAACTTGGAAATGAAACATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8268.18 Da        Isoelectric Point: 4.4000

>AT223101 WP_041465310.1 NZ_CP086345:c2777134-2776901 [Clavibacter michiganensis subsp. nebraskensis]
MAQNTSISLDGHFTGFLAREVESGRYRSASEVVRAGLRLLEDQETQLEALRAALVAGEASGEAAPFDLDAFIADKRA

Download         Length: 234 bp

>AT223101 NZ_CP118494:3067095-3067406 [Roseovarius tolerans]
ATGAAACATGAAATCAACGATTTCGATCCGGCTGAGTATCTCGACAACGATGAAATGATCGCCGGCTACCTATCGGATGC
GCTTGAAACGCAAGATCCCGCATTCATCGCTGATGCGATCGGTGTTGTTGCGCGAGCTAAAGGGATGAAGCGCGTCGCTG
AAGATGCAGGTGTTAGCCGCGAGAGCCTCTATCGTGCGCTGAGCGCCAACGGGAACCCTGAGTTCGGTACCGTGCTCAAG
GTATTGGCCTCGCTCAACATAAAGCTGATTGCGCAGCCTGATAGTGTCAAGCCGCATAACACGGCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M5BDX6

Antitoxin

Source ID Structure
AlphaFold DB A0A399PXI2

References