Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3839151..3839805 Replicon chromosome
Accession NZ_CP086342
Organism Escherichia coli strain ESY001

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C5N06_RS18425 Protein ID WP_000244777.1
Coordinates 3839151..3839558 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C5N06_RS18430 Protein ID WP_000354046.1
Coordinates 3839539..3839805 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N06_RS18405 (3835108) 3835108..3836841 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
C5N06_RS18410 (3836847) 3836847..3837557 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C5N06_RS18415 (3837582) 3837582..3838478 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C5N06_RS18420 (3838590) 3838590..3839111 + 522 WP_001055874.1 flavodoxin FldB -
C5N06_RS18425 (3839151) 3839151..3839558 - 408 WP_000244777.1 protein YgfX Toxin
C5N06_RS18430 (3839539) 3839539..3839805 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C5N06_RS18435 (3840048) 3840048..3841028 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C5N06_RS18440 (3841224) 3841224..3841883 - 660 WP_000250274.1 hemolysin III family protein -
C5N06_RS18445 (3842047) 3842047..3842358 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
C5N06_RS18450 (3842403) 3842403..3843836 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
C5N06_RS18455 (3843893) 3843893..3844636 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T223096 WP_000244777.1 NZ_CP086342:c3839558-3839151 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T223096 NZ_CP118438:309920-310219 [Vibrio vulnificus]
ATGCAAAAGAACAAATACAAGCTAAGTAACTTAGCGCAATCTCACTTGCGCAAAGCAAAAAACTACACTGTTGAAAACTT
TTCTGAATTACAGTGGCGCAACTACAAAGACACTCTGCTATCAGGGTTTCAAATGCTAGCTGATAACCCTGGGTTAGGAA
GAAGCTGTGACGAGATATACCCAAATGGCTTTTACTTCCCGATTGGAAAACACACCGCTTATTTCACTAAAGAAGATGGC
TTTATCTTAGTCGTAGCTGTCTTAGGTCAGTCACAGCTCCCACAGAATCATCTGAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT223096 WP_000354046.1 NZ_CP086342:c3839805-3839539 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT223096 NZ_CP118438:309670-309912 [Vibrio vulnificus]
ATGCATACATTAACAGCGAATGATGCCAAACGAAATTTCGGCGAACTACTTCTAAGCGCACAACGCGAGCCGGTAAAAAT
CAGCAAAAACAGTAAAGATACTGTCGTTGTCATGTCCATTCGTGACTTTGAAGAGTTAGAAGCTATGAAAGTTGAGTACC
TCAAACACTGCTTTAAGTCAGCTAAAGAAGACTTGAACAATGGAAACGCCATTGATGGCGAAAGCTTTCTAAATTCGCTG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References