Detailed information of TA system TA223083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2334021..2334659
• Replicon: chromosome
• Accession: NZ_CP086342
• Organism: Escherichia coli strain ESY001

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: C5N06_RS11310
• Protein ID: WP_001447010.1
• Coordinates: 2334021..2334197 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C5N06_RS11315
• Protein ID: WP_001270286.1
• Coordinates: 2334243..2334659 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N06_RS11290 (2329640)	2329640..2330854	-	1215	WP_001326689.1	BenE family transporter YdcO	-
C5N06_RS11295 (2330907)	2330907..2331443	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
C5N06_RS11300 (2331516)	2331516..2333477	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C5N06_RS11305 (2333569)	2333569..2333799	-	231	WP_000494244.1	YncJ family protein	-
C5N06_RS11310 (2334021)	2334021..2334197	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C5N06_RS11315 (2334243)	2334243..2334659	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C5N06_RS11320 (2334738)	2334738..2336144	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
C5N06_RS11325 (2336389)	2336389..2337534	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
C5N06_RS11330 (2337552)	2337552..2338565	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
C5N06_RS11335 (2338566)	2338566..2339507	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T223083 WP_001447010.1 NZ_CP086342:2334021-2334197 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T223083 NZ_CP118399:c92633-92346 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT223083 WP_001270286.1 NZ_CP086342:2334243-2334659 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT223083 NZ_CP118399:c92872-92633 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8