Detailed information of TA system TA022308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 12047..12563
• Replicon: plasmid pS18501
• Accession: NC_009661
• Organism: Shewanella baltica OS185

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B8EFR1
• Locus tag: SHEW185_RS22720
• Protein ID: WP_011979492.1
• Coordinates: 12279..12563 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B8EFR0
• Locus tag: SHEW185_RS22715
• Protein ID: WP_011979491.1
• Coordinates: 12047..12289 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHEW185_RS22690	7426..8585	+	1160	WP_086012318.1	IS3 family transposase	-
SHEW185_RS22695	8558..10060	-	1503	WP_011979488.1	hypothetical protein	-
SHEW185_RS22700	10310..10588	+	279	WP_157600332.1	hypothetical protein	-
SHEW185_RS22705	10735..11355	+	621	WP_011979489.1	DUF2913 family protein	-
SHEW185_RS22710	11448..11720	+	273	WP_011979490.1	hypothetical protein	-
SHEW185_RS22715	12047..12289	+	243	WP_011979491.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SHEW185_RS22720	12279..12563	+	285	WP_011979492.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SHEW185_RS22725	12762..13685	+	924	WP_011979493.1	IS5 family transposase	-
SHEW185_RS22730	13889..14917	+	1029	WP_011979494.1	IS110 family transposase	-
SHEW185_RS22745	16377..17416	-	1040	Protein_19	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..83224	83224
	-	inside	IScluster/Tn	-	-	4480..22499	18019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11079.11 Da
• Isoelectric Point: 10.5812

>T22308 WP_011979492.1 NC_009661:12279-12563 [Shewanella baltica OS185]
MIYKLVFDPRALKEWNKLGDPIRQQLKKKLAEILQHPHIGANKLRDLPDCYKIKLRTSGYRLVYQVQDERITVFVVAVGK
RERLAVYSTAEKRL

Nucleotide

• Length: 285 bp

>T22308 NC_009661:12279-12563 [Shewanella baltica OS185]
ATGATCTATAAGTTAGTATTTGATCCCCGCGCATTAAAGGAATGGAACAAGCTTGGAGATCCCATCAGGCAGCAGTTAAA
AAAGAAACTGGCCGAGATACTGCAACATCCTCATATCGGGGCTAACAAACTTAGAGATCTCCCTGACTGTTACAAAATCA
AGTTGCGTACATCAGGTTATCGCTTGGTATATCAAGTACAAGATGAACGCATTACCGTGTTTGTCGTGGCCGTAGGCAAG
CGTGAACGATTAGCGGTGTATTCAACAGCAGAAAAGCGCCTTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8816.24 Da
• Isoelectric Point: 4.4501

>AT22308 WP_011979491.1 NC_009661:12047-12289 [Shewanella baltica OS185]
MGTINIRIDDELKERSFAVLEKLGVSPSEALRQMLEYVATSGAMPFKPVLLTAEDEALISLVKERLASPRKGIEVSLDDL

Nucleotide

• Length: 243 bp

>AT22308 NC_009661:12047-12289 [Shewanella baltica OS185]
ATGGGCACTATTAATATTCGCATCGATGACGAGCTTAAGGAGCGTTCTTTTGCTGTGTTGGAAAAATTAGGCGTTAGTCC
CTCTGAGGCATTGCGGCAAATGCTTGAGTATGTCGCCACAAGCGGCGCTATGCCATTTAAACCCGTACTGCTAACAGCAG
AGGATGAAGCCTTGATCTCCTTGGTTAAAGAGCGTTTAGCGAGCCCTCGAAAGGGGATCGAGGTTTCGCTCGATGATCTA
TAA

Structures

Toxin

• AlphaFold DB: A9L6M1

Antitoxin

• AlphaFold DB: A9L6M0