223076

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4308803..4309300 Replicon chromosome
Accession NZ_CP086340
Organism Escherichia coli strain ESY003

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C5N08_RS20810 Protein ID WP_001173343.1
Coordinates 4308803..4308976 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C5N08_RS20815 Protein ID WP_000398619.1
Coordinates 4309004..4309300 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N08_RS20800 (4305473) 4305473..4306930 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
C5N08_RS20805 (4307167) 4307167..4308684 + 1518 WP_001295074.1 lysine--tRNA ligase -
C5N08_RS20810 (4308803) 4308803..4308976 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C5N08_RS20815 (4309004) 4309004..4309300 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C5N08_RS20820 (4309527) 4309527..4309799 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
C5N08_RS20825 (4309811) 4309811..4310041 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
C5N08_RS20830 (4310222) 4310222..4311853 + 1632 WP_001216477.1 sensor histidine kinase -
C5N08_RS20835 (4311850) 4311850..4312569 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T223076 WP_001173343.1 NZ_CP086340:c4308976-4308803 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T223076 NZ_CP118398:c4739976-4739692 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT223076 WP_000398619.1 NZ_CP086340:c4309300-4309004 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT223076 NZ_CP118398:c4740208-4739966 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References