Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3666628..3667282 Replicon chromosome
Accession NZ_CP086338
Organism Escherichia coli strain EJN001

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C5N09_RS17730 Protein ID WP_000244777.1
Coordinates 3666628..3667035 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C5N09_RS17735 Protein ID WP_000354046.1
Coordinates 3667016..3667282 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N09_RS17710 (3662585) 3662585..3664318 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
C5N09_RS17715 (3664324) 3664324..3665034 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C5N09_RS17720 (3665059) 3665059..3665955 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C5N09_RS17725 (3666067) 3666067..3666588 + 522 WP_001055874.1 flavodoxin FldB -
C5N09_RS17730 (3666628) 3666628..3667035 - 408 WP_000244777.1 protein YgfX Toxin
C5N09_RS17735 (3667016) 3667016..3667282 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C5N09_RS17740 (3667525) 3667525..3668505 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C5N09_RS17745 (3668701) 3668701..3669360 - 660 WP_000250274.1 hemolysin III family protein -
C5N09_RS17750 (3669524) 3669524..3669835 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
C5N09_RS17755 (3669880) 3669880..3671313 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
C5N09_RS17760 (3671370) 3671370..3672113 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T223048 WP_000244777.1 NZ_CP086338:c3667035-3666628 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T223048 NZ_CP118332:2579120-2579605 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCTCAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGAGAGCC
ATCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACACGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTATCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTCTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTTCGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAACGATTCTATTTACACCACGGATTCAAAGCCTCCACCACCCAGGAACGCACACTTTTCCTCGCGTTGCCT
AAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT223048 WP_000354046.1 NZ_CP086338:c3667282-3667016 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT223048 NZ_CP118332:2578866-2579132 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCACGGACCGACTTTATTCTTGAGATGGCCTGCCAGGCGGCAGAAAACGTCATTCTCGACCGCCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCACAATGGGAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References