Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4924366..4924974 Replicon chromosome
Accession NC_009656
Organism Pseudomonas aeruginosa PA7

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag PSPA7_RS22815 Protein ID WP_003114156.1
Coordinates 4924366..4924713 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag PSPA7_RS22820 Protein ID WP_003114155.1
Coordinates 4924723..4924974 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSPA7_RS22785 4919803..4920015 + 213 WP_012077061.1 cysteine-rich CWC family protein -
PSPA7_RS22790 4920015..4920707 + 693 WP_003151254.1 rRNA pseudouridine synthase -
PSPA7_RS22795 4920843..4921886 + 1044 WP_003151256.1 L,D-transpeptidase -
PSPA7_RS22800 4921966..4922703 + 738 WP_012077063.1 murein L,D-transpeptidase catalytic domain family protein -
PSPA7_RS22805 4923141..4924043 + 903 WP_012077064.1 alpha/beta hydrolase -
PSPA7_RS22815 4924366..4924713 - 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PSPA7_RS22820 4924723..4924974 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
PSPA7_RS31845 4925188..4925394 - 207 Protein_4587 tyrosine-type recombinase/integrase -
PSPA7_RS22825 4925404..4926567 - 1164 Protein_4588 IS3 family transposase -
PSPA7_RS22835 4927003..4927410 - 408 WP_033996869.1 hypothetical protein -
PSPA7_RS22845 4928260..4928517 + 258 WP_003148476.1 YjhX family toxin -
PSPA7_RS22850 4928581..4929087 + 507 WP_003148474.1 N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4925404..4926225 821


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T22304 WP_003114156.1 NC_009656:c4924713-4924366 [Pseudomonas aeruginosa PA7]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T22304 NC_009656:c4924713-4924366 [Pseudomonas aeruginosa PA7]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAACAATTGATCCG
CTATTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT22304 WP_003114155.1 NC_009656:c4924974-4924723 [Pseudomonas aeruginosa PA7]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT22304 NC_009656:c4924974-4924723 [Pseudomonas aeruginosa PA7]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References