Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1101499..1102117 Replicon chromosome
Accession NZ_CP086338
Organism Escherichia coli strain EJN001

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C5N09_RS05290 Protein ID WP_001291435.1
Coordinates 1101499..1101717 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C5N09_RS05295 Protein ID WP_000344800.1
Coordinates 1101743..1102117 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N09_RS05255 (1096788) 1096788..1097360 + 573 WP_000779831.1 YbaY family lipoprotein -
C5N09_RS05260 (1097391) 1097391..1097702 - 312 WP_000409911.1 MGMT family protein -
C5N09_RS05270 (1098081) 1098081..1098434 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C5N09_RS05275 (1098476) 1098476..1100026 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C5N09_RS05280 (1100190) 1100190..1100660 - 471 WP_000136192.1 YlaC family protein -
C5N09_RS05285 (1100776) 1100776..1101327 - 552 WP_000102564.1 maltose O-acetyltransferase -
C5N09_RS05290 (1101499) 1101499..1101717 - 219 WP_001291435.1 HHA domain-containing protein Toxin
C5N09_RS05295 (1101743) 1101743..1102117 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C5N09_RS05300 (1102663) 1102663..1105812 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
C5N09_RS05305 (1105835) 1105835..1107028 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223034 WP_001291435.1 NZ_CP086338:c1101717-1101499 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T223034 NZ_CP118278:c4642894-4642613 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGGGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT223034 WP_000344800.1 NZ_CP086338:c1102117-1101743 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT223034 NZ_CP118278:c4643126-4642884 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGGCTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References