Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 288111..288608 Replicon chromosome
Accession NZ_CP086338
Organism Escherichia coli strain EJN001

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C5N09_RS01310 Protein ID WP_001173343.1
Coordinates 288435..288608 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C5N09_RS01305 Protein ID WP_000398619.1
Coordinates 288111..288407 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N09_RS01285 (284842) 284842..285561 - 720 WP_000611288.1 two-component system response regulator DcuR -
C5N09_RS01290 (285558) 285558..287189 - 1632 WP_001216477.1 sensor histidine kinase -
C5N09_RS01295 (287370) 287370..287600 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
C5N09_RS01300 (287612) 287612..287884 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
C5N09_RS01305 (288111) 288111..288407 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C5N09_RS01310 (288435) 288435..288608 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C5N09_RS01315 (288727) 288727..290244 - 1518 WP_001295074.1 lysine--tRNA ligase -
C5N09_RS01320 (290481) 290481..291938 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T223030 WP_001173343.1 NZ_CP086338:288435-288608 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T223030 NZ_CP118278:794880-795290 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTCATCAACAGCGGAATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCGG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATGCCGGAGAGTGGCGGGACCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT223030 WP_000398619.1 NZ_CP086338:288111-288407 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT223030 NZ_CP118278:794633-794899 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References