Detailed information of TA system TA022303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4602238..4602824
• Replicon: chromosome
• Accession: NC_009656
• Organism: Pseudomonas aeruginosa PA7

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: PSPA7_RS21225
• Protein ID: WP_003120987.1
• Coordinates: 4602525..4602824 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: PSPA7_RS21220
• Protein ID: WP_003448662.1
• Coordinates: 4602238..4602528 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSPA7_RS21205	4597820..4599709	+	1890	WP_012076857.1	hypothetical protein	-
PSPA7_RS21210	4599706..4601682	+	1977	WP_012076858.1	DEAD/DEAH box helicase	-
PSPA7_RS21215	4601823..4602167	+	345	WP_003448665.1	hypothetical protein	-
PSPA7_RS21220	4602238..4602528	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
PSPA7_RS21225	4602525..4602824	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSPA7_RS21230	4603026..4604150	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
PSPA7_RS21235	4604150..4605859	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
PSPA7_RS21240	4605863..4607188	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
PSPA7_RS21245	4607178..4607711	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4573758..4659356	85598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T22303 WP_003120987.1 NC_009656:c4602824-4602525 [Pseudomonas aeruginosa PA7]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T22303 NC_009656:c4602824-4602525 [Pseudomonas aeruginosa PA7]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT22303 WP_003448662.1 NC_009656:c4602528-4602238 [Pseudomonas aeruginosa PA7]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT22303 NC_009656:c4602528-4602238 [Pseudomonas aeruginosa PA7]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73