Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1101866..1102484 Replicon chromosome
Accession NZ_CP086336
Organism Escherichia coli strain EBJ001

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C5N11_RS05290 Protein ID WP_001291435.1
Coordinates 1101866..1102084 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C5N11_RS05295 Protein ID WP_000344800.1
Coordinates 1102110..1102484 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N11_RS05255 (1097155) 1097155..1097727 + 573 WP_000779831.1 YbaY family lipoprotein -
C5N11_RS05260 (1097758) 1097758..1098069 - 312 WP_000409911.1 MGMT family protein -
C5N11_RS05270 (1098448) 1098448..1098801 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C5N11_RS05275 (1098843) 1098843..1100393 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C5N11_RS05280 (1100557) 1100557..1101027 - 471 WP_000136192.1 YlaC family protein -
C5N11_RS05285 (1101143) 1101143..1101694 - 552 WP_000102564.1 maltose O-acetyltransferase -
C5N11_RS05290 (1101866) 1101866..1102084 - 219 WP_001291435.1 HHA domain-containing protein Toxin
C5N11_RS05295 (1102110) 1102110..1102484 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C5N11_RS05300 (1103030) 1103030..1106179 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
C5N11_RS05305 (1106202) 1106202..1107395 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T223011 WP_001291435.1 NZ_CP086336:c1102084-1101866 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T223011 NZ_CP118274:c4688152-4687871 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAATCTCCAGCAGATTATGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT223011 WP_000344800.1 NZ_CP086336:c1102484-1102110 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT223011 NZ_CP118274:c4688384-4688142 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGTCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References