Detailed information of TA system TA223009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 502972..503548
• Replicon: chromosome
• Accession: NZ_CP086336
• Organism: Escherichia coli strain EBJ001

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: C5N11_RS02415
• Protein ID: WP_001297643.1
• Coordinates: 502972..503259 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: C5N11_RS02420
• Protein ID: WP_105310523.1
• Coordinates: 503246..503548 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N11_RS02400 (499208)	499208..500488	-	1281	WP_001037390.1	DUF445 domain-containing protein	-
C5N11_RS02405 (500529)	500529..501761	-	1233	WP_001137030.1	multidrug efflux MFS transporter MdtM	-
C5N11_RS02410 (502076)	502076..502773	+	698	Protein_464	IS1 family transposase	-
C5N11_RS02415 (502972)	502972..503259	+	288	WP_001297643.1	BrnT family toxin	Toxin
C5N11_RS02420 (503246)	503246..503548	+	303	WP_105310523.1	BrnA antitoxin family protein	Antitoxin
C5N11_RS02425 (503582)	503582..504538	-	957	WP_001297640.1	GTPase	-
C5N11_RS02430 (504549)	504549..504752	-	204	WP_000467859.1	YbdD/YjiX family protein	-
C5N11_RS02435 (504802)	504802..506952	-	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T223009 WP_001297643.1 NZ_CP086336:502972-503259 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T223009 NZ_CP118274:3965222-3965440 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11575.05 Da
• Isoelectric Point: 9.4651

>AT223009 WP_105310523.1 NZ_CP086336:503246-503548 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLHAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT223009 NZ_CP118274:3964821-3965195 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829GEV9