Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 289557..290054 Replicon chromosome
Accession NZ_CP086336
Organism Escherichia coli strain EBJ001

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C5N11_RS01315 Protein ID WP_001173343.1
Coordinates 289881..290054 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C5N11_RS01310 Protein ID WP_000398619.1
Coordinates 289557..289853 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N11_RS01290 (286288) 286288..287007 - 720 WP_000611288.1 two-component system response regulator DcuR -
C5N11_RS01295 (287004) 287004..288635 - 1632 WP_001216477.1 sensor histidine kinase -
C5N11_RS01300 (288816) 288816..289046 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
C5N11_RS01305 (289058) 289058..289330 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
C5N11_RS01310 (289557) 289557..289853 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C5N11_RS01315 (289881) 289881..290054 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C5N11_RS01320 (290173) 290173..291690 - 1518 WP_001295074.1 lysine--tRNA ligase -
C5N11_RS01325 (291927) 291927..293384 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T223007 WP_001173343.1 NZ_CP086336:289881-290054 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T223007 NZ_CP118274:1879647-1879749 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT223007 WP_000398619.1 NZ_CP086336:289557-289853 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT223007 NZ_CP118274:c1879753-1879606 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References