Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4491120..4491703 Replicon chromosome
Accession NZ_CP086334
Organism Escherichia coli strain EBJ003

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag C5N12_RS21745 Protein ID WP_000254738.1
Coordinates 4491368..4491703 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag C5N12_RS21740 Protein ID WP_000581937.1
Coordinates 4491120..4491368 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N12_RS21730 (4487459) 4487459..4488760 + 1302 WP_001521172.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
C5N12_RS21735 (4488808) 4488808..4491042 + 2235 WP_000226798.1 GTP diphosphokinase -
C5N12_RS21740 (4491120) 4491120..4491368 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
C5N12_RS21745 (4491368) 4491368..4491703 + 336 WP_000254738.1 endoribonuclease MazF Toxin
C5N12_RS21750 (4491775) 4491775..4492566 + 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
C5N12_RS21755 (4492794) 4492794..4494431 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
C5N12_RS21760 (4494518) 4494518..4495816 + 1299 WP_000036723.1 phosphopyruvate hydratase -
C5N12_RS21765 (4496009) 4496009..4496467 + 459 WP_000526135.1 IS200/IS605-like element IS200C family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4496009..4496467 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T223005 WP_000254738.1 NZ_CP086334:4491368-4491703 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T223005 NZ_CP118273:c4688167-4687886 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAATCTCCAGCAGATTATGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT223005 WP_000581937.1 NZ_CP086334:4491120-4491368 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT223005 NZ_CP118273:c4688399-4688157 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGTCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References