Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2877923..2878420 Replicon chromosome
Accession NZ_CP086334
Organism Escherichia coli strain EBJ003

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C5N12_RS14140 Protein ID WP_001173343.1
Coordinates 2877923..2878096 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C5N12_RS14145 Protein ID WP_000398619.1
Coordinates 2878124..2878420 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N12_RS14130 (2874593) 2874593..2876050 + 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -
C5N12_RS14135 (2876287) 2876287..2877804 + 1518 WP_000003806.1 lysine--tRNA ligase -
C5N12_RS14140 (2877923) 2877923..2878096 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C5N12_RS14145 (2878124) 2878124..2878420 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C5N12_RS14150 (2878649) 2878649..2878921 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
C5N12_RS14155 (2878933) 2878933..2879163 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
C5N12_RS14160 (2879344) 2879344..2880975 + 1632 WP_001216477.1 sensor histidine kinase -
C5N12_RS14165 (2880972) 2880972..2881691 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T222998 WP_001173343.1 NZ_CP086334:c2878096-2877923 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T222998 NZ_CP118272:c4618045-4617671 [Klebsiella quasipneumoniae]
ATGCAAAAACAATCCCTGTCCTCTCATAGGGCGGCCTCGTCACGTCTGTCATCAATCGAAATCTGGCGAAAATTACTGAA
GTATCTGCTGGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAGCACATTG
ATGCGGGAATCTCACTGTGTGATGCGGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCGTTTATCGGCAGCATTGATATGCTCCGCGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACAGGTGACAGATTCAGCGGAGGGAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT222998 WP_000398619.1 NZ_CP086334:c2878420-2878124 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT222998 NZ_CP118272:c4618454-4618095 [Klebsiella quasipneumoniae]
ATGAAGAAAGCAACCCGTGTAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
GGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAACAGATGGAGCTTATGCTGGCCAGCAGAGAACTCACG
CCCCACATTCAGCGCTGCATTACGCTCCACGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGCTCCTGTGGCTATCT
GTACATCGTAATTTATCCGACATCTGCAACAACCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References