Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2781080..2781675 Replicon chromosome
Accession NZ_CP086334
Organism Escherichia coli strain EBJ003

Toxin (Protein)


Gene name chpB Uniprot ID V0SXT4
Locus tag C5N12_RS13640 Protein ID WP_000239581.1
Coordinates 2781080..2781430 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID L4JJX7
Locus tag C5N12_RS13645 Protein ID WP_001223213.1
Coordinates 2781424..2781675 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N12_RS13620 (2776534) 2776534..2777556 - 1023 WP_001361374.1 ABC transporter permease -
C5N12_RS13625 (2777570) 2777570..2779072 - 1503 WP_001522399.1 sugar ABC transporter ATP-binding protein -
C5N12_RS13630 (2779205) 2779205..2780161 - 957 WP_001522398.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
C5N12_RS13635 (2780471) 2780471..2781001 + 531 WP_000055075.1 inorganic diphosphatase -
C5N12_RS13640 (2781080) 2781080..2781430 - 351 WP_000239581.1 endoribonuclease toxin ChpB Toxin
C5N12_RS13645 (2781424) 2781424..2781675 - 252 WP_001223213.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
C5N12_RS13650 (2781887) 2781887..2782228 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
C5N12_RS13655 (2782231) 2782231..2786010 - 3780 WP_001522396.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12453.41 Da        Isoelectric Point: 6.2206

>T222997 WP_000239581.1 NZ_CP086334:c2781430-2781080 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Download         Length: 351 bp

>T222997 NZ_CP118272:3965222-3965440 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9331.64 Da        Isoelectric Point: 4.6509

>AT222997 WP_001223213.1 NZ_CP086334:c2781675-2781424 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT222997 NZ_CP118272:3964821-3965195 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SXT4

Antitoxin

Source ID Structure
AlphaFold DB L4JJX7

References