Detailed information of TA system TA222993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2229250..2230939
• Replicon: chromosome
• Accession: NZ_CP086334
• Organism: Escherichia coli strain EBJ003

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: C5N12_RS10930
• Protein ID: WP_001521912.1
• Coordinates: 2229250..2230170 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: C5N12_RS10935
• Protein ID: WP_001521911.1
• Coordinates: 2230157..2230939 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N12_RS10915 (2225340)	2225340..2226467	-	1128	WP_069067521.1	MFS transporter	-
C5N12_RS10920 (2226609)	2226609..2227667	-	1059	WP_122452191.1	alpha/beta hydrolase	-
C5N12_RS10925 (2227932)	2227932..2228816	+	885	WP_001119037.1	LysR family transcriptional regulator	-
C5N12_RS10930 (2229250)	2229250..2230170	-	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
C5N12_RS10935 (2230157)	2230157..2230939	-	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
C5N12_RS10940 (2231255)	2231255..2234464	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
C5N12_RS10945 (2234811)	2234811..2235281	+	471	WP_000201185.1	hypothetical protein	-
C5N12_RS10950 (2235278)	2235278..2235380	+	103	Protein_2148	helix-turn-helix domain-containing protein	-
C5N12_RS10955 (2235472)	2235472..2235630	+	159	Protein_2149	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T222993 WP_001521912.1 NZ_CP086334:c2230170-2229250 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T222993 NZ_CP118271:c4688151-4687870 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAATCTCCAGCAGATTATGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT222993 WP_001521911.1 NZ_CP086334:c2230939-2230157 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT222993 NZ_CP118271:c4688383-4688141 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGTCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A829L165