Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2024550..2025168 Replicon chromosome
Accession NZ_CP086334
Organism Escherichia coli strain EBJ003

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C5N12_RS09975 Protein ID WP_001291435.1
Coordinates 2024950..2025168 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C5N12_RS09970 Protein ID WP_000344800.1
Coordinates 2024550..2024924 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N12_RS09960 (2019639) 2019639..2020832 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C5N12_RS09965 (2020855) 2020855..2024004 + 3150 WP_105313511.1 efflux RND transporter permease AcrB -
C5N12_RS09970 (2024550) 2024550..2024924 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C5N12_RS09975 (2024950) 2024950..2025168 + 219 WP_001291435.1 HHA domain-containing protein Toxin
C5N12_RS09980 (2025340) 2025340..2025891 + 552 WP_001522034.1 maltose O-acetyltransferase -
C5N12_RS09985 (2026007) 2026007..2026477 + 471 WP_001490888.1 YlaC family protein -
C5N12_RS09990 (2026641) 2026641..2028191 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C5N12_RS09995 (2028233) 2028233..2028586 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C5N12_RS10005 (2028965) 2028965..2029276 + 312 WP_000409911.1 MGMT family protein -
C5N12_RS10010 (2029307) 2029307..2029879 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T222992 WP_001291435.1 NZ_CP086334:2024950-2025168 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T222992 NZ_CP118271:c4618044-4617670 [Klebsiella quasipneumoniae]
ATGCAAAAACAATCCCTGTCCTCTCATAGGGCGGCCTCGTCACGTCTGTCATCAATCGAAATCTGGCGAAAATTACTGAA
GTATCTGCTGGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAGCACATTG
ATGCGGGAATCTCACTGTGTGATGCGGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCGTTTATCGGCAGCATTGATATGCTCCGCGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACAGGTGACAGATTCAGCGGAGGGAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT222992 WP_000344800.1 NZ_CP086334:2024550-2024924 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT222992 NZ_CP118271:c4618453-4618094 [Klebsiella quasipneumoniae]
ATGAAGAAAGCAACCCGTGTAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
GGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAACAGATGGAGCTTATGCTGGCCAGCAGAGAACTCACG
CCCCACATTCAGCGCTGCATTACGCTCCACGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGCTCCTGTGGCTATCT
GTACATCGTAATTTATCCGACATCTGCAACAACCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References