Detailed information of TA system TA222991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1600838..1601543
• Replicon: chromosome
• Accession: NZ_CP086334
• Organism: Escherichia coli strain EBJ003

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: C5N12_RS08110
• Protein ID: WP_000539521.1
• Coordinates: 1600838..1601224 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C5N12_RS08115
• Protein ID: WP_001280945.1
• Coordinates: 1601214..1601543 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N12_RS08090 (1596842)	1596842..1597468	+	627	WP_020233670.1	glutathione S-transferase GstB	-
C5N12_RS08095 (1597465)	1597465..1598580	-	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
C5N12_RS08100 (1598691)	1598691..1599074	-	384	WP_000497137.1	biofilm formation regulator BssR	-
C5N12_RS08105 (1599287)	1599287..1600612	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
C5N12_RS08110 (1600838)	1600838..1601224	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C5N12_RS08115 (1601214)	1601214..1601543	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
C5N12_RS08120 (1601613)	1601613..1602941	-	1329	WP_000086873.1	GGDEF domain-containing protein	-
C5N12_RS08125 (1602949)	1602949..1605297	-	2349	WP_021523051.1	EAL domain-containing protein	-
C5N12_RS08130 (1605475)	1605475..1606386	-	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T222991 WP_000539521.1 NZ_CP086334:1600838-1601224 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T222991 NZ_CP118271:3965222-3965440 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT222991 WP_001280945.1 NZ_CP086334:1601214-1601543 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT222991 NZ_CP118271:3964821-3965195 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6