Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1259654..1260438 Replicon chromosome
Accession NZ_CP086334
Organism Escherichia coli strain EBJ003

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag C5N12_RS06495 Protein ID WP_000613626.1
Coordinates 1259944..1260438 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag C5N12_RS06490 Protein ID WP_001110447.1
Coordinates 1259654..1259947 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N12_RS06480 (1254804) 1254804..1255763 - 960 WP_023153797.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
C5N12_RS06485 (1256336) 1256336..1259521 + 3186 WP_001522867.1 ribonuclease E -
C5N12_RS06490 (1259654) 1259654..1259947 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
C5N12_RS06495 (1259944) 1259944..1260438 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
C5N12_RS06500 (1260533) 1260533..1261486 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
C5N12_RS06505 (1261498) 1261498..1263141 - 1644 WP_001522865.1 flagellar hook-associated protein FlgK -
C5N12_RS06510 (1263207) 1263207..1264148 - 942 WP_001522863.1 flagellar assembly peptidoglycan hydrolase FlgJ -
C5N12_RS06515 (1264148) 1264148..1265245 - 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T222989 WP_000613626.1 NZ_CP086334:1259944-1260438 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T222989 NZ_CP118271:1879647-1879749 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT222989 WP_001110447.1 NZ_CP086334:1259654-1259947 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT222989 NZ_CP118271:c1879753-1879606 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References