Detailed information of TA system TA222987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 719584..720545
• Replicon: chromosome
• Accession: NZ_CP086334
• Organism: Escherichia coli strain EBJ003

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1Q5ZNB7
• Locus tag: C5N12_RS03760
• Protein ID: WP_021523084.1
• Coordinates: 719850..720545 (+)
• Length: 232 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: C5N12_RS03755
• Protein ID: WP_001296726.1
• Coordinates: 719584..719850 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5N12_RS03755 (719584)	719584..719850	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
C5N12_RS03760 (719850)	719850..720545	+	696	WP_021523084.1	HipA N-terminal domain-containing protein	Toxin
C5N12_RS03765 (720745)	720745..722097	-	1353	WP_001551127.1	SIR2 family protein	-
C5N12_RS03770 (722312)	722312..723344	-	1033	Protein_739	ISNCY family transposase	-
C5N12_RS03775 (723711)	723711..724610	-	900	WP_001523294.1	LysR family transcriptional regulator	-
C5N12_RS03780 (724749)	724749..725057	+	309	WP_001221046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	722312..723130	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 232 a.a.
• Molecular weight: 25773.79 Da
• Isoelectric Point: 9.9928

>T222987 WP_021523084.1 NZ_CP086334:719850-720545 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETIQAGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKI
YPRHFLATAKVLKFPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 696 bp

>T222987 NZ_CP118270:2652021-2652371 [Paenibacillus marchantiae]
TTGATCGTAAAACGCGGTGACGTTTTTTTTGCGGATCTTTCTCCCGTTGTCGGTTCCGAGCAAGGTGGAGTCAGGCCGGT
TCTGGTCATCCAGAATGACATCGGCAACCGGTTTAGTCCTACTTGTATTGTGGCGGCGATCACCGCCCAAATCCAAAAGG
CAAAGCTGCCGACGCATGTGGAGATTGATGCGGCGGCACACGGCTTTGACCGGGACTCGGTTATTTTGCTCGAACAAATA
CGGACGATTGATAAGCAGAGGCTGACCGACAAGATTACCCATCTGGACGAGGAGACCATGAAACTGGTCAACGAAGCCTT
ACAGATCAGCCTGGGTTTAATCGATTTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT222987 WP_001296726.1 NZ_CP086334:719584-719850 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT222987 NZ_CP118270:2651736-2652017 [Paenibacillus marchantiae]
GTGGCCAACTTGCAGAACACCAAGCGGATCATGATCAGTTTGCCTGATTATCTTTTGCAAGAAGTGGATGGCATCGTAGC
GCTGGAGAATTCCAACCGCAGCGAATTGATTAGGCAGGCCATGAAGCTGTATTTGACGGAGCGTAAGAAACGTTACATCC
GTGAATCGATGCAGCGAGGGTACATGGAGATGGCGAAGATTAACCTCACCATGGCATCCGAGGCCTTTCACGCGGAGGAA
GATGCGGACAGCACTCTGGACCGCTTAGTTAGCGAGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A1Q5ZNB7