Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 55170..55906 Replicon plasmid pKPN3
Accession NC_009649
Organism Klebsiella pneumoniae subsp. pneumoniae MGH 78578

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KPN_RS26490 Protein ID WP_003026803.1
Coordinates 55170..55652 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KPN_RS26495 Protein ID WP_003026799.1
Coordinates 55640..55906 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPN_RS26475 51329..52675 - 1347 WP_020314316.1 ISNCY family transposase -
KPN_RS26480 52905..53537 + 633 WP_001567369.1 hypothetical protein -
KPN_RS26485 53566..54969 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
KPN_RS26490 55170..55652 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KPN_RS26495 55640..55906 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KPN_RS26500 56082..56336 - 255 WP_004152108.1 hypothetical protein -
KPN_RS26505 56412..56669 - 258 WP_004152107.1 hypothetical protein -
KPN_RS26510 56718..56921 - 204 WP_004152106.1 hemolysin expression modulator Hha -
KPN_RS26515 56955..57323 - 369 WP_004152105.1 hypothetical protein -
KPN_RS26520 57367..57861 - 495 WP_004152104.1 DNA-binding protein -
KPN_RS26525 57892..58467 - 576 WP_004152103.1 hypothetical protein -
KPN_RS26530 58455..58724 - 270 WP_004152102.1 hypothetical protein -
KPN_RS26535 59082..59432 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KPN_RS26540 59482..59844 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..175879 175879


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T22297 WP_003026803.1 NC_009649:c55652-55170 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T22297 NC_009649:c55652-55170 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT22297 WP_003026799.1 NC_009649:c55906-55640 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT22297 NC_009649:c55906-55640 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References