Detailed information of TA system TA222969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 734207..734958
• Replicon: chromosome
• Accession: NZ_CP086332
• Organism: Xanthomonas translucens pv. phleipratensis strain LMG 843

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KFS85_RS03040
• Protein ID: WP_237652970.1
• Coordinates: 734482..734958 (+)
• Length: 159 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A0K2ZF15
• Locus tag: KFS85_RS03035
• Protein ID: WP_053837064.1
• Coordinates: 734207..734479 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFS85_RS03010 (KFS85_03015)	730224..730811	-	588	WP_237652966.1	restriction endonuclease subunit S	-
KFS85_RS03015 (KFS85_03020)	730960..731283	+	324	WP_237652967.1	hypothetical protein	-
KFS85_RS03020 (KFS85_03025)	731280..732263	-	984	WP_237652968.1	NAD(P)-dependent oxidoreductase	-
KFS85_RS03025 (KFS85_03030)	732357..732929	+	573	WP_144422966.1	ANTAR domain-containing protein	-
KFS85_RS03030 (KFS85_03035)	732937..734046	+	1110	WP_237652969.1	CmpA/NrtA family ABC transporter substrate-binding protein	-
KFS85_RS03035 (KFS85_03040)	734207..734479	+	273	WP_053837064.1	DUF1778 domain-containing protein	Antitoxin
KFS85_RS03040 (KFS85_03045)	734482..734958	+	477	WP_237652970.1	GNAT family N-acetyltransferase	Toxin
KFS85_RS03045 (KFS85_03050)	735572..736741	+	1170	WP_237653718.1	MFS transporter	-
KFS85_RS03050 (KFS85_03055)	736803..739220	+	2418	WP_237653719.1	nitrite reductase large subunit NirB	-
KFS85_RS03055 (KFS85_03060)	739224..739589	+	366	WP_003469752.1	nitrite reductase small subunit NirD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	726408..734958	8550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-89)

Sequences

Toxin

Protein

• Length: 159 a.a.
• Molecular weight: 17322.06 Da
• Isoelectric Point: 8.3749

>T222969 WP_237652970.1 NZ_CP086332:734482-734958 [Xanthomonas translucens pv. phleipratensis]
MSLTVPGPLNDVHRLDDFQCSAPELTRWLLERAHQNQLSGASRCFVVCDGQQRVVGYYALAAGVVAHEDAPGRIRRNMPN
PVPVIVLGRLAVHVDWAGQGVGRGLLKDAVQRTLQVCEQVGARALLCHAIDEAAKAFYLKHGFIASPIHDRTVMLPLR

Nucleotide

• Length: 477 bp

>T222969 NZ_CP118227:c85791-84448 [Proteus mirabilis]
ATGAAAGCTCAAATACTATCTGTTGCTATGAATGGTGATTTGGTTGGTAAACTAACCAAAAATACTGATGGTGGGATGTC
ATTTCAATACGCAGAAGAATGGTTGGCTGAACCAGGAGCGCGGGCAATCTCATTATCACTTCCTTTGCAAAAGGGGCGCA
TAATCGGTAGCGCAGTTTACAATTTCTTCAGCAACCTTTTACCTGATTCTGAGGCAATTATTGATAGAATGCAGATTCGT
TTTAAGGTAAAAACAAACCATCCTTTTGATCTGCTAGCCGCTGTAGGTCGTGACTGTGTTGGCGCAATCCAATTATATTC
TCCTGATACTGAAATTCCATCGGTGATGGAAACCCATGCTGAACCTTTAAATGAAAATCAAATCGAAGAGCTTTTAGACG
GTTATCAAACCGCCCCCCTTGGAATGTCCGAGGATGTAGACTTTCGGATCTCCCTTGCAGGGGCTCAAGAGAAAACAGCA
TTACTTTGGTATCAAGACTGTTGGCAGCGACCACAAGGCAGTACCCCGACAAGTCATATTTTCAAATTGCCCATAGGTCT
GATTGAACACCATAATATTGATCTGCGCGAAAGCTGTGAAAACGAATGGCTATGCTTGTGTATAGCAAGAGAATTTGGAT
TTCCAGTCACCAACGCTGAATTGGCAATATTCGGTCAGAAAAAGGTACTGATCGTAGAGCGTTTTGACCGAAAATGGTCC
TCATCTGGGAACTGGCTGTTGCGCCTTCCCCAAGAGGATTGCTGTCAGGCTTTGAGCGTTGCCCCTGCATTAAAATATGA
ATCTGATGGTGGTCCAGGTATTGAATCTATCATGACGTTGCTTTTGGGCTCACAAAAAGCCATCGACGATAGAGAAATGT
TTTTTAAATCACAAATTCTGTTCTGGATGCTGGCCGCTATAGATGGGCATGGGAAAAACTTTTCCATCTTCATTGAATCT
GGAACATCTTTTCGTTTAACGCCCTTGTATGATGTTATGTCTGCTTTCCCAATTTTTGAAGCACGAGGAATTGAGATAAA
AAAGGCCAAAATGGCTATGGCATTACAAGGAAAAAACAGACAATACAATTTTTCCATGATACAACCTCGTCATTTTATCT
CTACCGCTACAAAGGTCGGGTTTCCAACAAAAACCGCAATGGAGCTAATGGTTGATATGGCCGAAAAGACTGATGCAGTT
ATAGCTACCGTATCATCTCAACTACCTGCGGACTTTCCTGAGCATATCAGTAACGCCATATTTAGTGGGTTATCAATACA
AGCCGATAAGATTAAAAGATGGTCTTCAGGAACGAGCTCACAAAATATGATGGTTTCGAATTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9957.43 Da
• Isoelectric Point: 7.1666

>AT222969 WP_053837064.1 NZ_CP086332:734207-734479 [Xanthomonas translucens pv. phleipratensis]
MSAVTSKVINFRAPAAKQALIDHAVEVSGMNRTEFILDAVCEKAREVLADQTRFALSPQQLQRFNALLDAPLESNAAIRR
LLSTPAPWEH

Nucleotide

• Length: 273 bp

>AT222969 NZ_CP118227:c86046-85795 [Proteus mirabilis]
ATGAAAATTACAACGCCATCAATGTTAGCCGCTGCAATACATGACTTGCGCAAACAAAGTAAATTAACCCAAGCTCAAAC
GGCTGAACAAGTCGGTCTCAGGCAAGCAACTGTCTCTGAATTTGAGAACAACCCAGAATCAACAAAACTCGAAACGCTTT
TCAAAATCCTTGCCTCTTTGGAACTGGAACTACATGTAGTGAAGCGAGGAACAAACCTGAGTGAGGGTAAGGTTTGGAAT
AAGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0K2ZF15