Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4821620..4822262 Replicon chromosome
Accession NZ_CP086328
Organism Bacillus pacificus strain anQ-h4

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag LMD38_RS24780 Protein ID WP_000635963.1
Coordinates 4821620..4821970 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag LMD38_RS24785 Protein ID WP_000004570.1
Coordinates 4821975..4822262 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMD38_RS24765 (LMD38_24765) 4818556..4819014 - 459 WP_000344243.1 SprT family protein -
LMD38_RS24770 (LMD38_24770) 4819210..4819326 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
LMD38_RS24775 (LMD38_24775) 4819384..4821552 - 2169 WP_061183209.1 Tex family protein -
LMD38_RS24780 (LMD38_24780) 4821620..4821970 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LMD38_RS24785 (LMD38_24785) 4821975..4822262 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
LMD38_RS24790 (LMD38_24790) 4822562..4823731 - 1170 WP_000390608.1 alanine racemase -
LMD38_RS24795 (LMD38_24795) 4823850..4824800 - 951 WP_029141035.1 outer membrane lipoprotein carrier protein LolA -
LMD38_RS24800 (LMD38_24800) 4824957..4825316 - 360 WP_000635037.1 holo-ACP synthase -
LMD38_RS24805 (LMD38_24805) 4825409..4825981 + 573 WP_016716299.1 rhomboid family intramembrane serine protease -
LMD38_RS24810 (LMD38_24810) 4825974..4826936 - 963 WP_074585871.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T222968 WP_000635963.1 NZ_CP086328:c4821970-4821620 [Bacillus pacificus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T222968 NZ_CP118227:c14985-14602 [Proteus mirabilis]
ATGATATGGGTTAGCGCACAGGAAGTTATCGCTTTCCATGATCGTATATTACAACGTTTCCCAGGTGTGGTCGGTATGTC
CGATCCTGGCAGAGCGGAAGCTCTTATTTACCGGGTGCAAAACCGTAAACATTATGAAGGCATTACAGATGTGTTTGAAC
TGGCAGCAACTTATTGGGTCGCTATCGCTCGAGGGCACATATTTAATGACGGTAATAAACGTACCGCATTTTTTGTGACC
ATGACATTTCTCTATCGTAATGGCATCAGGATCCGCGATACTGGCAATATGCTGGAAAACTTAACAGTAGAAGCAGCTAC
AGGTGAGAAAACCGTCGACCAGTTAGCAAAACATCTACAAAACTTGGTAGAGAAAACTAACTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT222968 WP_000004570.1 NZ_CP086328:c4822262-4821975 [Bacillus pacificus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT222968 NZ_CP118227:c15203-14982 [Proteus mirabilis]
ATGCGTACATATACCTCGACACAAGCCCGAGCCAATATTTCAGAAGTATTAGATATTGCGACCCACGGTGAACCTGTTGA
AATCACCCGCAGAGACGGTTGTTCAGCGGTTGTTATCAGTAAAACTGAATTTGAGTCATACCAGAATGCTAAGCTAGATG
CAGAGTTTGATATGATGATGCAACGTCATGGACATACCGTAGAGGCGCTGACAAATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References