Detailed information of TA system TA222967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2751795..2752768
• Replicon: chromosome
• Accession: NZ_CP086328
• Organism: Bacillus pacificus strain anQ-h4

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: LMD38_RS14460
• Protein ID: WP_014893739.1
• Coordinates: 2752031..2752768 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A151UPU1
• Locus tag: LMD38_RS14455
• Protein ID: WP_000588714.1
• Coordinates: 2751795..2751923 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMD38_RS14435 (LMD38_14435)	2748646..2749251	-	606	Protein_2864	CatB-related O-acetyltransferase	-
LMD38_RS14440 (LMD38_14440)	2749452..2750921	-	1470	WP_016717520.1	beta-Ala-His dipeptidase	-
LMD38_RS14445 (LMD38_14445)	2751138..2751527	+	390	WP_016717519.1	YxeA family protein	-
LMD38_RS14450 (LMD38_14450)	2751546..2751722	-	177	WP_000852590.1	stage II sporulation protein SB	-
LMD38_RS14455 (LMD38_14455)	2751795..2751923	-	129	WP_000588714.1	hypothetical protein	Antitoxin
LMD38_RS14460 (LMD38_14460)	2752031..2752768	-	738	WP_014893739.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LMD38_RS14465 (LMD38_14465)	2753106..2753843	-	738	WP_000594137.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
LMD38_RS14470 (LMD38_14470)	2754010..2754492	-	483	WP_016717517.1	MarR family winged helix-turn-helix transcriptional regulator	-
LMD38_RS14475 (LMD38_14475)	2754600..2756285	+	1686	WP_062822446.1	alpha-keto acid decarboxylase family protein	-
LMD38_RS14480 (LMD38_14480)	2756463..2757245	-	783	WP_000381776.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.07 Da
• Isoelectric Point: 8.2998

>T222967 WP_014893739.1 NZ_CP086328:c2752768-2752031 [Bacillus pacificus]
VISNIRIGLFILAIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMVKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T222967 NZ_CP118223:c5700352-5699978 [Klebsiella grimontii]
ATGATTAAAGGCCCGGCGCTGTTTGATACTAATATTCTCATCGATTTATTCAGCGGTCGCGTTGAAGCGAAACAGGTTCT
GGAGGCTTATCCGCCACAGAATGCGATTAGCCTCATCACGTGGACGGAAGTAATGGTGGGGGCGAAAAAGTATCATCAGG
AACACCGCACGCGTATTGCACTGAGCGCGTTTAATATTATCGATATTTCACAGGATATTGCTGAACGAAGCGTCAATCTC
AGGAAGGAGTATGGGATGAAGCTGCCGGATGCCATCATACTGGCGACAGCGCAAATACATCGTTATGAACTGGTCACGCG
CAATACCAAAGATTTTTTCGGGATACCCGGAGTGATTACGCCTTATCATTTGTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5024.48 Da
• Isoelectric Point: 4.0932

>AT222967 WP_000588714.1 NZ_CP086328:c2751923-2751795 [Bacillus pacificus]
VIKDYDNDFFKKDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT222967 NZ_CP118223:c5700588-5700349 [Klebsiella grimontii]
ATGATGACGGGAACGAATATGGGCAGAATACTACTCGACTTATCGGATGACGTTATTAAACGTCTTGAAGACCTCAAACA
GCAGCGCAATCTTCCTCGAGCGGAACTGTTACGCGAGGCGGTTGAACAGTACCTGGAAAGACAAGCTGACACCACTATTT
CCAATGCGCTGGGGATTTGGCAAGGCTGTGAGGAAGAGGGAGTTGAATTCGAAAGGAAACTCCGCGAGGAGTGGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A151UPU1