Detailed information of TA system TA222965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 483818..484383
• Replicon: chromosome
• Accession: NZ_CP086326
• Organism: Lacticaseibacillus rhamnosus strain PMC203

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C2JYM4
• Locus tag: LL671_RS02245
• Protein ID: WP_005692155.1
• Coordinates: 484036..484383 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LL671_RS02240
• Protein ID: WP_020752297.1
• Coordinates: 483818..484036 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL671_RS02230 (LL671_02230)	480112..481902	+	1791	WP_005692150.1	ABC transporter ATP-binding protein	-
LL671_RS02235 (LL671_02235)	481889..483724	+	1836	WP_015764721.1	ABC transporter ATP-binding protein	-
LL671_RS02240 (LL671_02240)	483818..484036	+	219	WP_020752297.1	hypothetical protein	Antitoxin
LL671_RS02245 (LL671_02245)	484036..484383	+	348	WP_005692155.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LL671_RS02250 (LL671_02250)	485229..485897	+	669	WP_005692157.1	phenylalanine--tRNA ligase beta subunit-related protein	-
LL671_RS02255 (LL671_02255)	486138..486956	+	819	WP_014571588.1	ABC transporter permease subunit	-
LL671_RS02260 (LL671_02260)	486949..487650	+	702	WP_005692159.1	ATP-binding cassette domain-containing protein	-
LL671_RS02265 (LL671_02265)	487647..488888	+	1242	WP_005692160.1	acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13150.01 Da
• Isoelectric Point: 7.9817

>T222965 WP_005692155.1 NZ_CP086326:484036-484383 [Lacticaseibacillus rhamnosus]
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF

Nucleotide

• Length: 348 bp

>T222965 NZ_CP118223:4355258-4355476 [Klebsiella grimontii]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCCGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8117.33 Da
• Isoelectric Point: 5.7995

>AT222965 WP_020752297.1 NZ_CP086326:483818-484036 [Lacticaseibacillus rhamnosus]
MMDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE

Nucleotide

• Length: 219 bp

>AT222965 NZ_CP118223:4354857-4355231 [Klebsiella grimontii]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATTACTCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGTGCGGATCTGCAGAAATGGCGCAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7Z9DS75