222964

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 386824..387475 Replicon chromosome
Accession NZ_CP086326
Organism Lacticaseibacillus rhamnosus strain PMC203

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag LL671_RS01775 Protein ID WP_005686631.1
Coordinates 387092..387475 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag LL671_RS01770 Protein ID WP_005686632.1
Coordinates 386824..387072 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL671_RS01750 (LL671_01750) 381873..383261 + 1389 WP_014571607.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
LL671_RS01755 (LL671_01755) 383535..385043 + 1509 WP_005686635.1 DEAD/DEAH box helicase -
LL671_RS01760 (LL671_01760) 385212..385586 + 375 WP_005686634.1 holo-ACP synthase -
LL671_RS01765 (LL671_01765) 385573..386712 + 1140 WP_005691215.1 alanine racemase -
LL671_RS01770 (LL671_01770) 386824..387072 + 249 WP_005686632.1 antitoxin Antitoxin
LL671_RS01775 (LL671_01775) 387092..387475 + 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LL671_RS01780 (LL671_01780) 387989..388516 + 528 WP_005691221.1 QueT transporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T222964 WP_005686631.1 NZ_CP086326:387092-387475 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T222964 NZ_CP118223:2053651-2053754 [Klebsiella grimontii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT222964 WP_005686632.1 NZ_CP086326:386824-387072 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT222964 NZ_CP118223:c2053761-2053593 [Klebsiella grimontii]
AATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGTCCGTGACAAAAGTG
GACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References