Detailed information of TA system TA222962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2506270..2507518
• Replicon: chromosome
• Accession: NZ_CP086305
• Organism: Burkholderia ambifaria strain B21-004

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LMA00_RS27660
• Protein ID: WP_228891390.1
• Coordinates: 2506937..2507518 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LMA00_RS27655
• Protein ID: WP_228891389.1
• Coordinates: 2506270..2506827 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMA00_RS27630 (LMA00_27645)	2502157..2502792	+	636	WP_228891387.1	isochorismatase family cysteine hydrolase	-
LMA00_RS27635 (LMA00_27650)	2502872..2503603	-	732	WP_006752989.1	ribonuclease T2	-
LMA00_RS27640 (LMA00_27655)	2503785..2505188	-	1404	WP_228891388.1	DUF308 domain-containing protein	-
LMA00_RS27645 (LMA00_27660)	2505300..2505521	-	222	WP_043174164.1	hypothetical protein	-
LMA00_RS27650 (LMA00_27665)	2505551..2506198	+	648	WP_105786443.1	hypothetical protein	-
LMA00_RS27655 (LMA00_27670)	2506270..2506827	+	558	WP_228891389.1	helix-turn-helix transcriptional regulator	Antitoxin
LMA00_RS27660 (LMA00_27675)	2506937..2507518	+	582	WP_228891390.1	HD domain-containing protein	Toxin
LMA00_RS27665 (LMA00_27680)	2507619..2509157	-	1539	WP_228891391.1	MFS transporter	-
LMA00_RS27670 (LMA00_27685)	2509335..2510270	+	936	WP_105786447.1	LysR family transcriptional regulator	-
LMA00_RS27675 (LMA00_27690)	2510324..2511463	-	1140	WP_175724889.1	enoyl-CoA hydratase/isomerase family protein	-
LMA00_RS27680 (LMA00_27695)	2511460..2512251	-	792	WP_175724888.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-168)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21415.91 Da
• Isoelectric Point: 4.8450

>T222962 WP_228891390.1 NZ_CP086305:2506937-2507518 [Burkholderia ambifaria]
MNLDTIHARLDFLREAERLKDVLRSGHTSTGRSESTAEHSWRLCLMALVFADALPDVDTTKLLKLCVVHDLGEALHGDIP
AIEQAAHPDKSTHERDDLLTLTAGLDRALRNEIVALWDEYEAAESPEARAAKALDKLETILQHNQGSNPPDFDYAFNLGY
GRRYTDATPLFGAIRAIVDADTQRRIDARGDGT

Nucleotide

• Length: 582 bp

>T222962 NZ_CP118223:c1285024-1284650 [Klebsiella grimontii]
ATGCAAATACAATCCCTGTCCCCTCAAAGGGCGGCCTCGTCACGTCTGTCGTCAGTCGAAAGCTGGCGAAAATTGCTGGA
GTATCTGCTGAAACAACACTACGGTCTGATGATGAACGACACACCGTTTGGCGACGACAGTGTGATCCAGAAGAACATTG
ATGCGGGAATCTCACTGTGTGATGCGGTGAATTTTATCGTGGAAAAGTACGGTCTGGTGCGTACTGACCGTCACGGCTTC
AGTGTGACAGAGCAGTCTCCGTTCATCGACAGCATCGATATGCTCCGCGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGCTATCAAACCGTTACTGACATCACCAGCGGCAGATCCAGAGGAGGGAAATAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20324.32 Da
• Isoelectric Point: 10.7102

>AT222962 WP_228891389.1 NZ_CP086305:2506270-2506827 [Burkholderia ambifaria]
MTTSPLHTPPLGPQLKRWRALRRVKQSHAAELFGVAQSTISRWEAGIQQMSPDERATAERLLAARLDSAGDHALARLIAN
STAHMHLVCDLTHRLLACSPSRAAEFSRPLPMLLGTSLWRYASPEIVRMEAALDPLGWHDRAGPPSVEFTTGTNASRVVP
IRGSTCRWTRMTLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT222962 NZ_CP118223:c1285433-1285074 [Klebsiella grimontii]
ATGCAGAAAGCAACCCGTGTAATCAACCATAACATCGCGGAACCCTGGCGGGGCCTCAGACGCAACATCACACCGTGTTT
TGGTGCACGGCTGGTACAGGAGGGTAACCACCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGAAG
CAGATTTACGCTATCTGGACCAGGCATTTCCGGTACTGATGAAGCAAATGGAGCTTATGCTGGCCAGCAGCGAACTCACT
CCCCACATTCAGCGCTGCATCACGCTCCACGCAAAAGGGCTGATATGTGAGGCCGATACCCTCGGCTCCTGTGGCTATTT
GTTCATCGTAATTTATCCGACATCTGCAATAAGCGCATAA