Detailed information of TA system TA222961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-RelB
• Location: 2412321..2412844
• Replicon: chromosome
• Accession: NZ_CP086305
• Organism: Burkholderia ambifaria strain B21-004

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LMA00_RS27190
• Protein ID: WP_105786791.1
• Coordinates: 2412578..2412844 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B1YYI3
• Locus tag: LMA00_RS27185
• Protein ID: WP_012366507.1
• Coordinates: 2412321..2412581 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMA00_RS27165 (LMA00_27180)	2408393..2409781	+	1389	WP_006751722.1	MFS transporter	-
LMA00_RS27170 (LMA00_27185)	2410138..2411349	+	1212	WP_105786790.1	crosslink repair DNA glycosylase YcaQ family protein	-
LMA00_RS27175 (LMA00_27190)	2411452..2411670	-	219	WP_006751720.1	DUF1653 domain-containing protein	-
LMA00_RS27180 (LMA00_27195)	2411726..2412169	-	444	WP_006760724.1	GNAT family N-acetyltransferase	-
LMA00_RS27185 (LMA00_27200)	2412321..2412581	+	261	WP_012366507.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LMA00_RS27190 (LMA00_27205)	2412578..2412844	+	267	WP_105786791.1	Txe/YoeB family addiction module toxin	Toxin
LMA00_RS27195 (LMA00_27210)	2412964..2413587	+	624	WP_175752181.1	HAD hydrolase-like protein	-
LMA00_RS27200 (LMA00_27215)	2413966..2414538	+	573	WP_105786792.1	DUF4142 domain-containing protein	-
LMA00_RS27205 (LMA00_27220)	2414658..2415257	-	600	WP_175671206.1	GNAT family protein	-
LMA00_RS27210 (LMA00_27225)	2415345..2416259	-	915	WP_228891359.1	LysR family transcriptional regulator	-
LMA00_RS27215 (LMA00_27230)	2416369..2417607	+	1239	WP_175654464.1	diaminopimelate decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10438.80 Da
• Isoelectric Point: 9.4575

>T222961 WP_105786791.1 NZ_CP086305:2412578-2412844 [Burkholderia ambifaria]
MSVRRVLFTSDARDDYVYWQGQDRKTLKRINQLIREAQRTPFEGIGKPEPLKANLSGFWSRRIDDTNRLVYEADDLQISI
VSCRYHYA

Nucleotide

• Length: 267 bp

>T222961 NZ_CP118223:846654-847064 [Klebsiella grimontii]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGCGCGCAGTGGTTTTCACTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTAGTGTTCGACTGCG
TACGCAGCCAGCGGCGGATCCATGCCCGTCAGGGCGAAATAAAGCTGCTGATCGATTCGCGTCTGCGCTGGCAAAAGGCG
GAGTGGGAGATTATCGGTACGCCGTGGGTAATCAACAGCGGCATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGTAC
CCAGCATTTATGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGCGACCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGAGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9759.03 Da
• Isoelectric Point: 6.0735

>AT222961 WP_012366507.1 NZ_CP086305:2412321-2412581 [Burkholderia ambifaria]
MRTIHFSDARGNLKTVIDQVVDDADVTLITRRDAPNAVIMSQEYYDSLMETVHLLRSPANVAHLERSIAQLRKGKAKEHK
LAEDDE

Nucleotide

• Length: 261 bp

>AT222961 NZ_CP118223:846407-846673 [Klebsiella grimontii]
ATGGACATTAACAATAAAGCCCGTATCCATTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGATACGCTAAGCGATGGCGATAAGGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TCTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCTGAACTGCAGCGGATGGTAAAATTAATTCAGACTCGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9KUE4