Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 5195819..5196629 Replicon chromosome
Accession NC_009648
Organism Klebsiella pneumoniae subsp. pneumoniae MGH 78578

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KPN_RS25575 Protein ID WP_002887280.1
Coordinates 5196096..5196629 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A2X3FK06
Locus tag KPN_RS25570 Protein ID WP_032428116.1
Coordinates 5195819..5196085 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPN_RS25545 (KPN_04738) 5191690..5192034 - 345 WP_015959364.1 cation efflux system protein CusF -
KPN_RS25550 (KPN_04739) 5192052..5193437 - 1386 WP_032428115.1 efflux transporter outer membrane subunit -
KPN_RS25560 (KPN_04740) 5193610..5194293 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
KPN_RS25565 (KPN_04741) 5194283..5195716 + 1434 WP_015959366.1 Cu(+)/Ag(+) sensor histidine kinase -
KPN_RS25570 5195819..5196085 + 267 WP_032428116.1 DUF1778 domain-containing protein Antitoxin
KPN_RS25575 (KPN_04742) 5196096..5196629 + 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
KPN_RS25580 (KPN_04743) 5196677..5197798 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T22296 WP_002887280.1 NC_009648:5196096-5196629 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T22296 NC_009648:5196096-5196629 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTTATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9980.46 Da        Isoelectric Point: 10.1757

>AT22296 WP_032428116.1 NC_009648:5195819-5196085 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQAWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT22296 NC_009648:5195819-5196085 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAAGCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
AlphaFold DB A0A2X3FK06

References