Detailed information of TA system TA222956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 30255..30820
• Replicon: chromosome
• Accession: NZ_CP086305
• Organism: Burkholderia ambifaria strain B21-004

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LMA00_RS16450
• Protein ID: WP_175797956.1
• Coordinates: 30449..30820 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1FGI9
• Locus tag: LMA00_RS16445
• Protein ID: WP_006752328.1
• Coordinates: 30255..30452 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMA00_RS16420 (LMA00_16420)	25562..25954	-	393	WP_175671801.1	nuclear transport factor 2 family protein	-
LMA00_RS16425 (LMA00_16425)	26127..26612	+	486	WP_228891487.1	isochorismatase family protein	-
LMA00_RS16430 (LMA00_16430)	26681..28234	-	1554	WP_006752325.1	galactarate dehydratase	-
LMA00_RS16435 (LMA00_16435)	28463..29377	+	915	WP_006752326.1	5-dehydro-4-deoxyglucarate dehydratase	-
LMA00_RS16440 (LMA00_16440)	29514..30065	-	552	WP_006752327.1	hypothetical protein	-
LMA00_RS16445 (LMA00_16445)	30255..30452	+	198	WP_006752328.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LMA00_RS16450 (LMA00_16450)	30449..30820	+	372	WP_175797956.1	type II toxin-antitoxin system VapC family toxin	Toxin
LMA00_RS16455 (LMA00_16455)	30954..32531	-	1578	WP_228891488.1	aldehyde dehydrogenase (NADP(+))	-
LMA00_RS16460 (LMA00_16460)	32812..34179	+	1368	WP_175671812.1	MFS transporter	-
LMA00_RS16465 (LMA00_16465)	34241..34966	-	726	WP_228891489.1	site-2 protease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13460.62 Da
• Isoelectric Point: 6.3272

>T222956 WP_175797956.1 NZ_CP086305:30449-30820 [Burkholderia ambifaria]
MILVDTSVWIDHLRAGDATLATLLEAERVLIHPFIIGELALGSMRDRQTVLDALRDLPAAAPATDDEVQRMIDVVPLHGL
GIGYVDAHLLASVRLTSGSKLWTRDRRLLAAAERLQLVARPGH

Nucleotide

• Length: 372 bp

>T222956 NZ_CP118183:c13523-13254 [Entomospira culicis]
ATGACTTACACGCTACATTATACGACTGTCGCGATCAAGGCGTTAAAAAAACTTGATCGTCCCAGCCAAATCTTGATCAT
GAATTGGATCGAGAAGCATTTGATCGATTGCGAAGATCCTCGCAGTAAGGGCAAAGCGTTGAAGGCGAATTTATCGGGGG
CATGGCGGTATCGTATCGGTGATTACCGTATTATCGCTGAGATCGATGATAGCACGGTTACAATTTTGTTGTTGGCGATT
GGGCACCGAAAAGCCATCTATAATAAATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7054.10 Da
• Isoelectric Point: 10.6826

>AT222956 WP_006752328.1 NZ_CP086305:30255-30452 [Burkholderia ambifaria]
MRTTLSLDDALLAKAQQLTGVTEKSALVREALRALIARESARRLARLGGTEPDLESVPRRPSEPA

Nucleotide

• Length: 198 bp

>AT222956 NZ_CP118183:c13744-13523 [Entomospira culicis]
ATGACGATATCGATCCGTTTTAACGATGAAGAAGCCGAATTAATCAAACAATACGCTAAGCACAAAAAAACCACCGTATC
ACAAGTGATGCGAGAGAGCATCCTAGCCGAACTAGAAGATGAGTATGACATTGATGCCTACCAACAAGCGATGCAAGCCT
ACAAAGAGCATCCTATTACGCATAACCATGACGAAGTCAAAAAGCTATTGGATTTAGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2S9KMB8