Detailed information of TA system TA222940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 308076..308715
• Replicon: chromosome
• Accession: NZ_CP086298
• Organism: Burkholderia ambifaria strain B21-006

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LL998_RS17525
• Protein ID: WP_175741337.1
• Coordinates: 308299..308715 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q0B8R6
• Locus tag: LL998_RS17520
• Protein ID: WP_006753828.1
• Coordinates: 308076..308312 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL998_RS17505 (LL998_17515)	303574..304443	+	870	WP_175659163.1	LysR substrate-binding domain-containing protein	-
LL998_RS17510 (LL998_17520)	305170..306396	-	1227	WP_229081983.1	acyltransferase	-
LL998_RS17515 (LL998_17525)	306623..307825	+	1203	WP_229081984.1	glycosyltransferase family 87 protein	-
LL998_RS17520 (LL998_17530)	308076..308312	+	237	WP_006753828.1	DNA-binding protein	Antitoxin
LL998_RS17525 (LL998_17535)	308299..308715	+	417	WP_175741337.1	type II toxin-antitoxin system VapC family toxin	Toxin
LL998_RS17530 (LL998_17540)	309290..309493	+	204	WP_006751843.1	cold-shock protein	-
LL998_RS17535 (LL998_17545)	309955..310218	+	264	WP_006751842.1	translation initiation factor IF-1	-
LL998_RS17540 (LL998_17550)	310512..310625	-	114	Protein_276	4-hydroxy-2-oxovalerate aldolase	-
LL998_RS17545 (LL998_17555)	310621..310887	+	267	Protein_277	FAD-binding protein	-
LL998_RS17550 (LL998_17560)	310891..311405	+	515	Protein_278	transferase hexapeptide repeat family protein	-
LL998_RS17555 (LL998_17565)	311682..311930	+	249	WP_175705882.1	hypothetical protein	-
LL998_RS17560 (LL998_17570)	311954..313117	-	1164	WP_229081986.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	292006..322664	30658

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15265.46 Da
• Isoelectric Point: 6.9900

>T222940 WP_175741337.1 NZ_CP086298:308299-308715 [Burkholderia ambifaria]
VFLIDTNVISEIRKGKRANRGVRAFFRQAEADASPLYLSVVTVAELRRGVDLIRHRGDHPQASALEAWMATILSGYASNI
LPVDIETAQMWGHLRVPDPAHALDHLIAATALINDLTVVTRNVDDFARTGVRLLNPFD

Nucleotide

• Length: 417 bp

>T222940 NZ_CP118163:1013829-1013984 [Staphylococcus hyicus]
ATGTCTGAAGAAAAACATATCGAACATGAAAATGAGATGGTAGATAATTTCGATGATCTTGTGCAATTAGGCAAAGAAAT
GGAACAGATTTCTGAAACAAATGATCAAGACAAATTGAACCAATCACATGACTCTGAGGGACGTTCAAATCAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8682.73 Da
• Isoelectric Point: 5.0774

>AT222940 WP_006753828.1 NZ_CP086298:308076-308312 [Burkholderia ambifaria]
MANLLVRNVDDSIVQSLREQAAANGRSAEAEHRAILAEALGRPKRRTFAQVLMSMPDVGEDADFQRVQDSGEVRRVFD

Nucleotide

• Length: 237 bp

>AT222940 NZ_CP118163:1013211-1013810 [Staphylococcus hyicus]
ATGGCAATGAACTTTAAAGTTTTTAAAGACAAAGATACTGCAGCGATTTATGCGGCGGATATCATTAGAAAACAATTCAA
TAATAATCCAACAACGATCGCAGGGTTTCATTTAAATGAGGAAGCGGCACCAGTGCTTGATTATTTAAAGCGTAATGTTG
ATGACCATGCGGTTGATTTTAGTCAAATTCACATTTTAGATTACGATAAACAAACATCATACTTTAAAGCATTAGGTGTA
CCAGAAAAACAAATTCATGAAATTCCAGAAGAAGATGAAGTTGAAAAATTTATTGAACGTAAAGCGAAGACGAAAGACAA
TAAAGGCAAACTCACATTGCAAGTTGTTTCAATCAATAATAAAGGTGAATTTGGTATTCCTGTTACGAATGGTTTAAAGC
CTGCACGTGAAATTTTTGTTGTTGTGACAGGAAGTGAAAAAGCGGATGTCATTAAAAAATTATATGAAGACAATGGTAAT
ACAAGCTTTATTCCTTCAAGTTTAAAAGCTCACCGTATGGTCAATGTCATTCTTGATGAAGCTGCTGCGCAAGGGTTACC
TGATGATGTAAGAGCGTACTTCACTTCACTTTATGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2S9KQ31