Detailed information of TA system TA222938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 58449..60214
• Replicon: chromosome
• Accession: NZ_CP086298
• Organism: Burkholderia ambifaria strain B21-006

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LL998_RS16410
• Protein ID: WP_229081779.1
• Coordinates: 58449..59360 (+)
• Length: 304 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: LL998_RS16415
• Protein ID: WP_229081780.1
• Coordinates: 59357..60214 (+)
• Length: 286 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL998_RS16380 (LL998_16390)	53810..54889	-	1080	WP_229081776.1	porin	-
LL998_RS16385 (LL998_16395)	55340..55657	+	318	WP_006751284.1	non-heme iron oxygenase ferredoxin subunit	-
LL998_RS16390 (LL998_16400)	55659..55970	+	312	WP_229081777.1	hypothetical protein	-
LL998_RS16395 (LL998_16405)	56025..57095	+	1071	WP_229081778.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
LL998_RS16400 (LL998_16410)	57088..57858	+	771	WP_105833376.1	SDR family oxidoreductase	-
LL998_RS16405 (LL998_16415)	57902..58429	+	528	WP_012366322.1	cupin domain-containing protein	-
LL998_RS16410 (LL998_16420)	58449..59360	+	912	WP_229081779.1	alpha/beta fold hydrolase	Toxin
LL998_RS16415 (LL998_16425)	59357..60214	+	858	WP_229081780.1	IclR family transcriptional regulator	Antitoxin
LL998_RS16420 (LL998_16430)	60211..61008	+	798	WP_229081781.1	SDR family oxidoreductase	-
LL998_RS16425 (LL998_16435)	61001..62668	+	1668	WP_105833380.1	thiamine pyrophosphate-binding protein	-
LL998_RS16430 (LL998_16440)	62661..63488	+	828	WP_229081782.1	aspartate dehydrogenase	-
LL998_RS16435 (LL998_16445)	63587..65077	+	1491	WP_175659023.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (56-284)

Antitoxin

• Transcription regulator IclR, N-terminal (24-72)

Sequences

Toxin

Protein

• Length: 304 a.a.
• Molecular weight: 31268.41 Da
• Isoelectric Point: 7.3664

>T222938 WP_229081779.1 NZ_CP086298:58449-59360 [Burkholderia ambifaria]
MSVIDKRERDRTAAYAALLGQFPEQRCAAGAAGTIGYREAGAGEAGAQHAGHALPVVLLHGIGSGAASWVGQLDTLGASR
RVLAWDAPGYGVSTPVHGASPTAADYAASLNAWLEALGIERCVLVGHSLGAIIAGGLCRLRPARIAGLLLISPAGGYGRA
PAETRDTRRDARLAMLDELGPAGLAEQRSGNMLSGFASEDARAWVRWNMARIVPAGYAQATHLLANADLATDLAGFRGRT
AVAVGANDAITPPAACERIAAAAQVELQVIPQAGHAGYVEAPAVYSSLIDTFCRQCDTQRGRA

Nucleotide

• Length: 912 bp

>T222938 NZ_CP118163:861845-862078 [Staphylococcus hyicus]
ATGCAAATTGATCGTGTGTTTGTAGAGGGATTAGATGAGGAACAACAACGAACAGATTTAACACAACGTTTGAATCATAT
GATAGGGGTTAAAGGAGTAAGTATAGATGGTCACTTACAATGTGTAACACTTAAATATGAAACGCCAATGAATTTAAATA
CGTTAGAAAAAGAAATTTACGATGCTGGTTTTAAAGTATTACGAACAGTAAAAGGAGAGATTACGAATGAGTAA

Antitoxin

Protein

• Length: 286 a.a.
• Molecular weight: 31399.89 Da
• Isoelectric Point: 6.3573

>AT222938 WP_229081780.1 NZ_CP086298:59357-60214 [Burkholderia ambifaria]
MSEPLQQPESDSTKADAGYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYK
LGIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQCQSPVFSAIRVNVGTRLPAHATTHGH
VLMGDLSLKELRALYPEGTLSRMTSATPETVDALYEVIREDALRGYGVSNSSFERGISVVTAPVRNDTQRIVACITVTVP
RPEIDEALIADGLFDKVQRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 858 bp

>AT222938 NZ_CP118163:861545-861832 [Staphylococcus hyicus]
ATGAATGACCATGCGCATCATTCTGAAGAGATTAAAAAGAACTTAACGTCACGTTTGAATAGAATCGAGGGACAAGTTCG
TGCGATAAATCGCATGATTGATGAAGATGTTTATTGTGACGATGTATTGACACAAATTAGAGCAACACGTTCTGCATTAA
ACAGTGTTGCTACAAAGCTACTCGATTATCACATGAAAGGTTGTATTACAAAGAAAATTGAGGATGGACATGAAACTGAA
GCAATGGAAGAATTGTTGGTCACATTTCAAAAGTTAATGAAAGATTAA