Detailed information of TA system TA222933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 893297..893936
• Replicon: chromosome
• Accession: NZ_CP086295
• Organism: Burkholderia sp. B21-007

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LMA01_RS20685
• Protein ID: WP_228907750.1
• Coordinates: 893520..893936 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q0B8R6
• Locus tag: LMA01_RS20680
• Protein ID: WP_006753828.1
• Coordinates: 893297..893533 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMA01_RS20650 (LMA01_20660)	888415..888897	+	483	WP_228907756.1	glycosyl hydrolase 108 family protein	-
LMA01_RS20655 (LMA01_20665)	888894..889445	+	552	WP_228907755.1	3TM-type holin	-
LMA01_RS20660 (LMA01_20670)	889448..889687	+	240	WP_229050799.1	hypothetical protein	-
LMA01_RS20665 (LMA01_20675)	890658..891305	+	648	WP_228907754.1	hypothetical protein	-
LMA01_RS20670 (LMA01_20680)	891318..892544	-	1227	WP_229050800.1	acyltransferase	-
LMA01_RS20675 (LMA01_20685)	892842..893045	+	204	WP_228907751.1	hypothetical protein	-
LMA01_RS20680 (LMA01_20690)	893297..893533	+	237	WP_006753828.1	DNA-binding protein	Antitoxin
LMA01_RS20685 (LMA01_20695)	893520..893936	+	417	WP_228907750.1	type II toxin-antitoxin system VapC family toxin	Toxin
LMA01_RS20690 (LMA01_20700)	894169..895065	+	897	WP_228907749.1	VOC family protein	-
LMA01_RS20695 (LMA01_20705)	895075..895968	+	894	WP_175672647.1	CoA transferase subunit A	-
LMA01_RS20700 (LMA01_20710)	895979..896776	+	798	WP_228907748.1	ketoacid CoA transferase	-
LMA01_RS20705 (LMA01_20715)	896785..897711	+	927	WP_228907747.1	enoyl-CoA hydratase	-
LMA01_RS20710 (LMA01_20720)	897708..898826	+	1119	WP_228907746.1	nitronate monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15274.46 Da
• Isoelectric Point: 6.8809

>T222933 WP_228907750.1 NZ_CP086295:893520-893936 [Burkholderia sp. B21-007]
VFLIDTNVISEIRKGKRTNRGVRAFFRQAEADASPLYLSVVTVGELRRGVDLIRHRGDHPQASALEAWMATILSGYASNI
LPVDLETTQMWGHLRVPDPAHALDKLIAATALINDLTVVTRNVDDFARTGVQLLNPFD

Nucleotide

• Length: 417 bp

>T222933 NZ_CP118156:c2534064-2533771 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCGATGGCACACCACGCTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATACAGGCGCCGGCTACCGCCTCTATTTCACGGTGCGCGGCCACACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8682.73 Da
• Isoelectric Point: 5.0774

>AT222933 WP_006753828.1 NZ_CP086295:893297-893533 [Burkholderia sp. B21-007]
MANLLVRNVDDSIVQSLREQAAANGRSAEAEHRAILAEALGRPKRRTFAQVLMSMPDVGEDADFQRVQDSGEVRRVFD

Nucleotide

• Length: 237 bp

>AT222933 NZ_CP118156:c2533769-2533479 [Pseudomonas chlororaphis]
ATGTCCGACACCCTGCTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAGTACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCGCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGAGCCAAGCCGCGTTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCTCGCGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9KQ31