Detailed information of TA system TA222932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 613634..615384
• Replicon: chromosome
• Accession: NZ_CP086295
• Organism: Burkholderia sp. B21-007

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LMA01_RS19315
• Protein ID: WP_228907975.1
• Coordinates: 613634..614530 (+)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: LMA01_RS19320
• Protein ID: WP_228907974.1
• Coordinates: 614527..615384 (+)
• Length: 286 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMA01_RS19285 (LMA01_19295)	608996..610075	-	1080	WP_228907977.1	porin	-
LMA01_RS19290 (LMA01_19300)	610524..610841	+	318	WP_006751284.1	non-heme iron oxygenase ferredoxin subunit	-
LMA01_RS19295 (LMA01_19305)	610843..611154	+	312	WP_006758061.1	hypothetical protein	-
LMA01_RS19300 (LMA01_19310)	611209..612279	+	1071	WP_011659097.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
LMA01_RS19305 (LMA01_19315)	612272..613042	+	771	WP_228907976.1	SDR family oxidoreductase	-
LMA01_RS19310 (LMA01_19320)	613087..613614	+	528	WP_006751280.1	cupin domain-containing protein	-
LMA01_RS19315 (LMA01_19325)	613634..614530	+	897	WP_228907975.1	alpha/beta fold hydrolase	Toxin
LMA01_RS19320 (LMA01_19330)	614527..615384	+	858	WP_228907974.1	IclR family transcriptional regulator	Antitoxin
LMA01_RS19325 (LMA01_19335)	615381..616178	+	798	WP_228907973.1	SDR family oxidoreductase	-
LMA01_RS19330 (LMA01_19340)	616171..617838	+	1668	WP_166723280.1	thiamine pyrophosphate-binding protein	-
LMA01_RS19335 (LMA01_19345)	617831..618658	+	828	WP_006758069.1	aspartate dehydrogenase	-
LMA01_RS19340 (LMA01_19350)	618765..620255	+	1491	WP_228907972.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-279)

Antitoxin

• Transcription regulator IclR, C-terminal (144-266)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30773.04 Da
• Isoelectric Point: 7.3667

>T222932 WP_228907975.1 NZ_CP086295:613634-614530 [Burkholderia sp. B21-007]
MSVIDKRERDRTAAFAALLGQFPEQRCAAGAAGTIGYREAGAQHAGHALPVVLLHGIGSGAASWVCQLDTLGASRRVLAW
DAPGYGVSTPVHGASPTAADYAASLNAWLEALGIERCVLVGHSLGAIIAGGLSRLMPARIAGLLLISPAGGYGRAPAETR
DTRRDARLAMLEALGPAGLAEQRSGNMLSGFASEDARAWVRWNMARIVPAGYAQATHLLANADLATDLAGFRGRTAVAVG
ANDAITPPAACERIAAAAQVELQVIPQAGHAGYVEAPAVYSSLIDAFCRQCDTQRGMA

Nucleotide

• Length: 897 bp

>T222932 NZ_CP118156:c2533769-2533479 [Pseudomonas chlororaphis]
ATGTCCGACACCCTGCTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAGTACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCGCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGAGCCAAGCCGCGTTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCTCGCGGCTGA

Antitoxin

Protein

• Length: 286 a.a.
• Molecular weight: 31299.82 Da
• Isoelectric Point: 6.6518

>AT222932 WP_228907974.1 NZ_CP086295:614527-615384 [Burkholderia sp. B21-007]
MSEPLQQPESESTKADAGYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYK
LGIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQCQSPGFSAIRVNVGTRLPAHATTHGH
VLMGDLSLKELRALYPEGTLSRMTSATPETVDALYEVIREDALRGYGISNSSFERGISVVTAPVRNDTQKIVACITVTVP
RPEIDAALIADGLFDKVQRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 858 bp

>AT222932 NZ_CP118156:c2534064-2533771 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCGATGGCACACCACGCTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATACAGGCGCCGGCTACCGCCTCTATTTCACGGTGCGCGGCCACACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA