Detailed information of TA system TA222928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 197238..198964
• Replicon: chromosome
• Accession: NZ_CP086294
• Organism: Burkholderia sp. B21-007

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: LMA01_RS00890
• Protein ID: WP_042302661.1
• Coordinates: 197238..197888 (+)
• Length: 217 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: LMA01_RS00895
• Protein ID: WP_229050472.1
• Coordinates: 197888..198964 (+)
• Length: 359 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMA01_RS00870 (LMA01_00870)	192567..192971	-	405	WP_229050468.1	DUF3396 domain-containing protein	-
LMA01_RS00875 (LMA01_00875)	193070..193393	+	324	WP_229050469.1	helix-turn-helix domain-containing protein	-
LMA01_RS00880 (LMA01_00880)	193432..195972	+	2541	WP_229050470.1	N-6 DNA methylase	-
LMA01_RS00885 (LMA01_00885)	195965..197245	+	1281	WP_229050471.1	restriction endonuclease subunit S	-
LMA01_RS00890 (LMA01_00890)	197238..197888	+	651	WP_042302661.1	DUF4433 domain-containing protein	Toxin
LMA01_RS00895 (LMA01_00895)	197888..198964	+	1077	WP_229050472.1	macro domain-containing protein	Antitoxin
LMA01_RS00900 (LMA01_00900)	198974..202237	+	3264	WP_229050473.1	HsdR family type I site-specific deoxyribonuclease	-
LMA01_RS00905 (LMA01_00905)	202237..202944	+	708	WP_229050474.1	SprT family zinc-dependent metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (16-216)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 217 a.a.
• Molecular weight: 25067.61 Da
• Isoelectric Point: 7.5410

>T222928 WP_042302661.1 NZ_CP086294:197238-197888 [Burkholderia sp. B21-007]
MANHYPNLNPDKALIWRIVHRDNLQWILDNGLHCGNSAVKAPGWVSIGNPELIDKRANHPVPRPPGGLLNDYVPFYFTPF
SPMLRNIHTGWGGIQKRSNEEIVILVSSLFHIVGQGMPFLFTDSHAYYQWANFYSDVAYLDKIDWPLLQRRDFKRDPDDP
AKFERYQAEALIHQHLPVSGLLGVVCYTEQLKQRIEQEIQVRNLNLPVYARTGWYF

Nucleotide

• Length: 651 bp

>T222928 NZ_CP118156:c541233-540805 [Pseudomonas chlororaphis]
ATGATCGTGCTCGACACCAATGTGCTGTCGGAACTGATGCGTCCCCAACCGGACGCATCGGTATTGCTGTGGATCGAAGC
GCAACCTGTCGAAGACCTCTACATCAGCGCCATGACCATGGCCGAGATTCTTCACGGCATCGCGCGCCTGCCCCACGGCA
GGCGCAAACAGATGCTCCATGCGTCGGCCCTGGCGATGTTCGAGGAAGATTTCATCGAACGCATCGTACCCTTCGGGACC
CAGGCGGCTCTTTACTACGCCCATCTGATTTCGCACCGGGAAAGCCTGGGGCGTCCGATCAGCCTGGTGGATGCGCAAAT
TGCGGCCATATGCCGCGTCCATGACGCGGCGATCGCCACCCGTAACGCCAAGGACTTCAGCGACACCGGGTTGATCGTTA
TCAACCCCTGGCTGCCACTCGAGGTTTGA

Antitoxin

Protein

• Length: 359 a.a.
• Molecular weight: 40529.50 Da
• Isoelectric Point: 6.2649

>AT222928 WP_229050472.1 NZ_CP086294:197888-198964 [Burkholderia sp. B21-007]
MITFTQGNLLEARVEALVNTVNTVGVMGKGIALMFKERFDENFRRYSAACKAKEVRTGKMFVTPVHELDGPRWIVNFPTK
QHWRAPSRMEWIIEGLQDLRQFLIEQQVKSIAIPPLGAGNGGLEWADVREQIELTLGDLDIDIVVFEPTKQYQNVAKRAG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIERYNPGDNPLNLQFAPHKYGPYANRLDHLLNNLDGSYLH
SQKRISDADPLDVIWFDDERKTFVQTYLKSEAKAYTQALESTAALIDGFESPFGMELLATVDWLLAREGVAPTVPALREG
LRHWRGGPDAGARKDRLFDDRTLGIALERLTQAAPAIA

Nucleotide

• Length: 1077 bp

>AT222928 NZ_CP118156:c541475-541230 [Pseudomonas chlororaphis]
ATGGCCAGCCTGACAATCCGAAACCTGGACGACCACATCAAAGAGCGCCTGCGCATCGAGGCCGCCCACAACGGTCACTC
CATGGAAGAAGAAGCCCGCTTGATTTTGCGCCGCGCCCTGAACAGAACAACCGACAGCCAAGGCCTGGCCAGTCGTATCC
GGGCACGCTTTGCCGAGGCCGGCGGAGTGAAGCTTGAGCTGCCCGAACGTACCGAGCCGGCACGCGTGGTGGATCTGGGC
GAATGA