Detailed information of TA system TA222922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 747603..748249
• Replicon: chromosome
• Accession: NZ_CP086292
• Organism: Burkholderia ambifaria strain B21-008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LL999_RS19325
• Protein ID: WP_229063622.1
• Coordinates: 747842..748249 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q0BA09
• Locus tag: LL999_RS19320
• Protein ID: WP_011658485.1
• Coordinates: 747603..747845 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL999_RS19305 (LL999_19325)	743600..745006	+	1407	WP_229063619.1	aspartate ammonia-lyase	-
LL999_RS19310 (LL999_19330)	745106..746599	+	1494	WP_229063620.1	amino acid permease	-
LL999_RS19315 (LL999_19335)	746644..747342	-	699	WP_229063621.1	phospholipase	-
LL999_RS19320 (LL999_19340)	747603..747845	+	243	WP_011658485.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LL999_RS19325 (LL999_19345)	747842..748249	+	408	WP_229063622.1	type II toxin-antitoxin system VapC family toxin	Toxin
LL999_RS19330 (LL999_19350)	748262..748648	-	387	WP_229063623.1	hypothetical protein	-
LL999_RS19335 (LL999_19355)	748745..751135	+	2391	WP_229063624.1	MCP four helix bundle domain-containing protein	-
LL999_RS19340 (LL999_19360)	751138..751878	+	741	WP_011658488.1	methyl-accepting chemotaxis protein	-
LL999_RS19345 (LL999_19365)	751875..752471	+	597	WP_229063625.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14868.97 Da
• Isoelectric Point: 6.4877

>T222922 WP_229063622.1 NZ_CP086292:747842-748249 [Burkholderia ambifaria]
MKFLLDTNAVIAILKGEQAMLARLRSHQPADFGIPAIVAHELYYGAYKSQRAAANLARVEGLQFEVVSFDAEDAQHAGEI
RAQLAAVGTPIGPYDALIAGQARARRLVLVTHNTREFERISELQVEDWLAAAREK

Nucleotide

• Length: 408 bp

>T222922 NZ_CP118155:c2612929-2612747 [Pseudomonas chlororaphis]
GTGCAAAGCAGACAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGTAGCCATCATCTGTT
CAAGCATCCCTACCGGCCCGAGACCGTTCCGGTGCCTCACCCGAAGAAAGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8872.03 Da
• Isoelectric Point: 4.7628

>AT222922 WP_011658485.1 NZ_CP086292:747603-747845 [Burkholderia ambifaria]
METAKIFKHGGSQAVRLPKDFRFDTTEVRIRRHGASVILEPVPQDWAWLTPLIGPVDADFEAAAATEPAAQERPGLDVFE

Nucleotide

• Length: 243 bp

>AT222922 NZ_CP118155:c2612711-2612310 [Pseudomonas chlororaphis]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCGGGCGCGGT
AACCGCCGGCGACACCTTCGAGGCCGCGTATAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAGCTGGCCCGTG
AGGGGCAAGCCATTCCCCTGCCCTCGCCCACCGGCACGCACCGCGCCAACCCCGACTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAAACCGAGAAGGTCAATGTGACCCTGCCGGGTTACGTGATTCAACGAAT
CGACCGCTTTGTGCGGGAGCACAACATCAAGAGCCGATCGTCATTCCTGGCGGATGCGGCGATGGAAAAGCTGGGGCGTT
AA

Structures

Antitoxin

• AlphaFold DB: A0A3P0NDJ4