Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4585670..4586295 Replicon chromosome
Accession NC_009648
Organism Klebsiella pneumoniae subsp. pneumoniae MGH 78578

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KPN_RS22570 Protein ID WP_002882817.1
Coordinates 4585912..4586295 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KPN_RS22565 Protein ID WP_004150355.1
Coordinates 4585670..4585912 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPN_RS22540 (KPN_04180) 4581661..4582260 + 600 WP_004151865.1 glucose-1-phosphatase -
KPN_RS22545 (KPN_04181) 4582254..4583114 + 861 WP_004146232.1 virulence factor BrkB family protein -
KPN_RS22550 (KPN_04182) 4583111..4583548 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
KPN_RS22555 (KPN_04183) 4583593..4584534 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KPN_RS22560 (KPN_04184) 4584548..4585465 - 918 WP_015959199.1 alpha/beta hydrolase -
KPN_RS22565 (KPN_04185) 4585670..4585912 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
KPN_RS22570 (KPN_04186) 4585912..4586295 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KPN_RS22575 (KPN_04187) 4586469..4587398 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KPN_RS22580 (KPN_04188) 4587395..4588030 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KPN_RS22585 (KPN_04189) 4588027..4588929 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T22292 WP_002882817.1 NC_009648:4585912-4586295 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T22292 NC_009648:4585912-4586295 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT22292 WP_004150355.1 NC_009648:4585670-4585912 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT22292 NC_009648:4585670-4585912 [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References