Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4897434..4898050 Replicon chromosome
Accession NZ_CP086287
Organism Citrobacter freundii strain DY2010

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag LMH92_RS23585 Protein ID WP_003028682.1
Coordinates 4897434..4897808 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag LMH92_RS23590 Protein ID WP_043018956.1
Coordinates 4897808..4898050 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH92_RS23570 (4894937) 4894937..4895839 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
LMH92_RS23575 (4895836) 4895836..4896471 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
LMH92_RS23580 (4896468) 4896468..4897397 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
LMH92_RS23585 (4897434) 4897434..4897808 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH92_RS23590 (4897808) 4897808..4898050 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
LMH92_RS23595 (4898256) 4898256..4899164 + 909 WP_044701816.1 alpha/beta hydrolase -
LMH92_RS23600 (4899315) 4899315..4900256 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
LMH92_RS23605 (4900301) 4900301..4900738 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
LMH92_RS23610 (4900735) 4900735..4901607 - 873 WP_003028669.1 virulence factor BrkB family protein -
LMH92_RS23615 (4901601) 4901601..4902200 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T222917 WP_003028682.1 NZ_CP086287:c4897808-4897434 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T222917 NZ_CP118154:4735981-4736274 [Pseudomonas chlororaphis]
ATGAACGAACCGCTATTCGACTATGACCCCGCCACGGCCTTGCGCGACCCGCAAGCCATCGCCCTGTTCATGGCGGACGC
CTTCGAAACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGTGTGGTGGCGCGGGCCAAGGGCATGACTGAGCTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTATCGCTCATTCAGCGAAAAAGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGTGCACTGGGACTGGACATGACGGCGCGGGTGCATGGGGCGTCTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT222917 WP_043018956.1 NZ_CP086287:c4898050-4897808 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT222917 NZ_CP118154:4735685-4735984 [Pseudomonas chlororaphis]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATTGC
TGCGCGGATTTTTCGCCTGGCCAACGGTCTTGCCGGGGACGTGTCCCCGGTGGGCGAAGGCGTCAGCGAGTTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCGCGGCTTGCAACTCGTCATCCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAATACCTGGCCCGTCGTTGGAGCCGATCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References