Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 4745500..4746166 Replicon chromosome
Accession NZ_CP086287
Organism Citrobacter freundii strain DY2010

Toxin (Protein)


Gene name tad Uniprot ID A0A0D7LIM5
Locus tag LMH92_RS22900 Protein ID WP_044702260.1
Coordinates 4745807..4746166 (-) Length 120 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID A0A0D7LIR0
Locus tag LMH92_RS22895 Protein ID WP_003844682.1
Coordinates 4745500..4745817 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH92_RS22865 (4741167) 4741167..4741991 + 825 WP_003031641.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
LMH92_RS22870 (4742060) 4742060..4743283 + 1224 WP_044702265.1 L-sorbose 1-phosphate reductase -
LMH92_RS22875 (4743285) 4743285..4744097 + 813 WP_044702264.1 shikimate 5-dehydrogenase -
LMH92_RS22880 (4744149) 4744149..4744505 - 357 WP_032948294.1 hypothetical protein -
LMH92_RS22885 (4744647) 4744647..4744808 + 162 WP_003841416.1 phage protein -
LMH92_RS22890 (4745212) 4745212..4745490 + 279 WP_044702262.1 putative addiction module antidote protein -
LMH92_RS22895 (4745500) 4745500..4745817 - 318 WP_003844682.1 helix-turn-helix transcriptional regulator Antitoxin
LMH92_RS22900 (4745807) 4745807..4746166 - 360 WP_044702260.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LMH92_RS22905 (4746380) 4746380..4747069 + 690 WP_003841421.1 dipeptidase PepE -
LMH92_RS22910 (4747135) 4747135..4748766 - 1632 WP_032938230.1 Na/Pi cotransporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(14-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-90)


Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 13437.49 Da        Isoelectric Point: 10.2555

>T222916 WP_044702260.1 NZ_CP086287:c4746166-4745807 [Citrobacter freundii]
MTKPLYWVGQARKDLIAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Download         Length: 360 bp

>T222916 NZ_CP118154:4735685-4735984 [Pseudomonas chlororaphis]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATTGC
TGCGCGGATTTTTCGCCTGGCCAACGGTCTTGCCGGGGACGTGTCCCCGGTGGGCGAAGGCGTCAGCGAGTTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCGCGGCTTGCAACTCGTCATCCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAATACCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11466.35 Da        Isoelectric Point: 9.0005

>AT222916 WP_003844682.1 NZ_CP086287:c4745817-4745500 [Citrobacter freundii]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Download         Length: 318 bp

>AT222916 NZ_CP118154:4735981-4736274 [Pseudomonas chlororaphis]
ATGAACGAACCGCTATTCGACTATGACCCCGCCACGGCCTTGCGCGACCCGCAAGCCATCGCCCTGTTCATGGCGGACGC
CTTCGAAACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGTGTGGTGGCGCGGGCCAAGGGCATGACTGAGCTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTATCGCTCATTCAGCGAAAAAGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGTGCACTGGGACTGGACATGACGGCGCGGGTGCATGGGGCGTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7LIM5

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LIR0

References