Detailed information of TA system TA222915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4489368..4489884
• Replicon: chromosome
• Accession: NZ_CP086287
• Organism: Citrobacter freundii strain DY2010

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LMH92_RS21680
• Protein ID: WP_044699148.1
• Coordinates: 4489368..4489652 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0J1MV10
• Locus tag: LMH92_RS21685
• Protein ID: WP_003839576.1
• Coordinates: 4489642..4489884 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH92_RS21665 (4485693)	4485693..4486277	+	585	WP_003839584.1	fructose PTS transporter subunit IIA	-
LMH92_RS21670 (4486655)	4486655..4488793	+	2139	WP_003025782.1	anaerobic ribonucleoside-triphosphate reductase	-
LMH92_RS21675 (4488900)	4488900..4489364	+	465	WP_032937736.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
LMH92_RS21680 (4489368)	4489368..4489652	-	285	WP_044699148.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH92_RS21685 (4489642)	4489642..4489884	-	243	WP_003839576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LMH92_RS21690 (4489962)	4489962..4491875	-	1914	WP_003839574.1	BglG family transcription antiterminator	-
LMH92_RS21695 (4491897)	4491897..4492637	-	741	WP_044699149.1	KDGP aldolase family protein	-
LMH92_RS21700 (4492634)	4492634..4493752	-	1119	WP_044699152.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
LMH92_RS21705 (4493736)	4493736..4494869	-	1134	WP_016149471.1	amidohydrolase/deacetylase family metallohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10865.69 Da
• Isoelectric Point: 10.1988

>T222915 WP_044699148.1 NZ_CP086287:c4489652-4489368 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVTVFVVAVGK
RQHSAVYLDANKRL

Nucleotide

• Length: 285 bp

>T222915 NZ_CP118154:c3752734-3752453 [Pseudomonas chlororaphis]
ATGACTTATAAGCTCCAGTTTCTACCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTCATACCCTACGCGAGCAATTCAA
GAAGAAGCTGGCCGAGCGCCTGGAAATGCCACGGGTGCCCGCGGATGCCCTTCACGGCATGGCGGACTGCTACAAGATCA
AGCTCAAAGCCTCTGGTTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTCGTGGTTTCGGTGGTGGCCGTGGGTAAA
CGTGAACGCAGTGCCGTGTATGAGCGGGCACGAAAGCGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8961.34 Da
• Isoelectric Point: 5.0651

>AT222915 WP_003839576.1 NZ_CP086287:c4489884-4489642 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT222915 NZ_CP118154:c3752969-3752724 [Pseudomonas chlororaphis]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAATCCTTCGGCTGTCCTGAGCAGCGCACA
GGGTGGTCCGGTTGCGGTGCTCAATCACAATCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCGATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGTCACGAGACCCCGGTCCCGGTAAATCTGGATGAC
TTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MV10