222914

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4036765..4037444 Replicon chromosome
Accession NZ_CP086287
Organism Citrobacter freundii strain DY2010

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag LMH92_RS19585 Protein ID WP_003031349.1
Coordinates 4037103..4037444 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag LMH92_RS19580 Protein ID WP_003031347.1
Coordinates 4036765..4037082 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH92_RS19555 (4032858) 4032858..4034855 - 1998 WP_016245729.1 choline BCCT transporter BetT -
LMH92_RS19560 (4035403) 4035403..4035550 + 148 Protein_3843 hypothetical protein -
LMH92_RS19565 (4035564) 4035564..4036025 + 462 WP_003031344.1 antirestriction protein -
LMH92_RS19570 (4036041) 4036041..4036517 + 477 WP_003031345.1 RadC family protein -
LMH92_RS19575 (4036526) 4036526..4036747 + 222 WP_003031346.1 DUF987 domain-containing protein -
LMH92_RS19580 (4036765) 4036765..4037082 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LMH92_RS19585 (4037103) 4037103..4037444 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
LMH92_RS19590 (4037981) 4037981..4038169 - 189 WP_213311616.1 hypothetical protein -
LMH92_RS19595 (4038220) 4038220..4038693 - 474 WP_213311614.1 DUF2280 domain-containing protein -
LMH92_RS19600 (4038786) 4038786..4038965 - 180 WP_071681666.1 hypothetical protein -
LMH92_RS19605 (4038962) 4038962..4039624 - 663 WP_191608710.1 Gp138 family membrane-puncturing spike protein -
LMH92_RS19610 (4039635) 4039635..4039982 - 348 WP_191608711.1 hypothetical protein -
LMH92_RS19615 (4039987) 4039987..4041078 - 1092 WP_213311612.1 hypothetical protein -
LMH92_RS19620 (4041084) 4041084..4041251 - 168 WP_047601733.1 hypothetical protein -
LMH92_RS19625 (4041232) 4041232..4041420 - 189 WP_224776483.1 hypothetical protein -
LMH92_RS19630 (4041433) 4041433..4041996 - 564 WP_044702595.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4035564..4050380 14816


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T222914 WP_003031349.1 NZ_CP086287:4037103-4037444 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T222914 NZ_CP118154:c3647493-3647311 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAGTA
CAAGTATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT222914 WP_003031347.1 NZ_CP086287:4036765-4037082 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT222914 NZ_CP118154:c3647281-3646880 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGCCATACCGCGGCAGAAGCCTTCGAGAATGTGAAAGAGGCCTTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAACCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAACCCCGATTACGCCGGTGGCGTGTGGGGCATGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGCTTCAACGCCACCCTGCCGGAGCAATTGCTGGAACGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGCTCGCGTTCGGGCTTTCTCGCCGCGGCGGCATTGCGCGAGTTATCGGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References