Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3801111..3801731 Replicon chromosome
Accession NZ_CP086287
Organism Citrobacter freundii strain DY2010

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LMH92_RS18485 Protein ID WP_002892050.1
Coordinates 3801513..3801731 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag LMH92_RS18480 Protein ID WP_003021733.1
Coordinates 3801111..3801485 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH92_RS18470 (3796258) 3796258..3797451 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LMH92_RS18475 (3797474) 3797474..3800623 + 3150 WP_003021736.1 efflux RND transporter permease AcrB -
LMH92_RS18480 (3801111) 3801111..3801485 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
LMH92_RS18485 (3801513) 3801513..3801731 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LMH92_RS18490 (3802038) 3802038..3802589 + 552 WP_044699372.1 maltose O-acetyltransferase -
LMH92_RS18495 (3802706) 3802706..3803176 + 471 WP_003021724.1 YlaC family protein -
LMH92_RS18500 (3803255) 3803255..3803395 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
LMH92_RS18505 (3803397) 3803397..3803657 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
LMH92_RS18510 (3803846) 3803846..3805399 + 1554 WP_044699371.1 EAL domain-containing protein -
LMH92_RS18515 (3805451) 3805451..3805804 - 354 WP_003831033.1 DUF1428 family protein -
LMH92_RS18520 (3805869) 3805869..3806498 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T222913 WP_002892050.1 NZ_CP086287:3801513-3801731 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T222913 NZ_CP118154:c2978975-2978697 [Pseudomonas chlororaphis]
GTGAGGGAATTGAAATGGACGAGCAAGGCACTGTCGGACCTGGCCCGACTGTTTGAGTTTCTTGCTCCGGTGAACCGTTC
GGTGGCAGCTCGAACTGTGCAATCGCTCACGCAGGCCCCGGATACCTTGCTGACCAATCCGCGTATCGGCGAACAGTTGG
AGGAGTTTCAGCCTCGCGATGTTCGGCGTTTATTGGTCGGGCCTTACGAGATGCGTTACGAGATCCAGGATGCGACGCTC
TACATCCTGCGAGTGTGGCATGTACGAGAAGACCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT222913 WP_003021733.1 NZ_CP086287:3801111-3801485 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT222913 NZ_CP118154:c2979235-2978972 [Pseudomonas chlororaphis]
ATGACCGCAACTAAAGTGCTGACCGCACATGTGCCGTTGGAGTTGGCCGACAAGGTCGACCAGATGGCCTCTCGGCTGGA
GCGCTCGCGCGGCTGGATCGTCAAGCAGGCTCTGGTGGCGTGGGTCGATCAGGAAGAGGAACGCAGTCGCCTGACCCATG
AGGCTTTGGCCGATGTGGATGTTGGGCGGGTCATCGATCACCAGGCGGTCCAGGCATGGGCTGAAAGCCTGATGAGTGAC
AAGCCTTTGCCGGTGCCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References