Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 867020..867674 Replicon chromosome
Accession NZ_CP086287
Organism Citrobacter freundii strain DY2010

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag LMH92_RS04250 Protein ID WP_003026936.1
Coordinates 867267..867674 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag LMH92_RS04245 Protein ID WP_003026938.1
Coordinates 867020..867286 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH92_RS04220 (862221) 862221..863654 - 1434 WP_044700805.1 6-phospho-beta-glucosidase BglA -
LMH92_RS04225 (863775) 863775..864503 - 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
LMH92_RS04230 (864556) 864556..864867 + 312 WP_044700802.1 N(4)-acetylcytidine aminohydrolase -
LMH92_RS04235 (865030) 865030..865689 + 660 WP_003838267.1 hemolysin III family protein -
LMH92_RS04240 (865783) 865783..866763 - 981 WP_003026944.1 tRNA-modifying protein YgfZ -
LMH92_RS04245 (867020) 867020..867286 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
LMH92_RS04250 (867267) 867267..867674 + 408 WP_003026936.1 protein YgfX Toxin
LMH92_RS04255 (867719) 867719..868240 - 522 WP_003026933.1 flavodoxin FldB -
LMH92_RS04260 (868354) 868354..869250 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
LMH92_RS04265 (869274) 869274..869987 + 714 WP_003026923.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LMH92_RS04270 (869993) 869993..871726 + 1734 WP_044700800.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T222904 WP_003026936.1 NZ_CP086287:867267-867674 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T222904 NZ_CP118153:c1871058-1870876 [Pseudomonas chlororaphis]
GTGGACAGTCGGTATTTGATCGGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGAGGCAGTCACCATCATTT
CAGGCATCCCAGTAAACCGGGGCTGGTGACAGTGCCACATCCCAAAAAGGACCTGCTGAGAAAAACCGCCATCAGCATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT222904 WP_003026938.1 NZ_CP086287:867020-867286 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT222904 NZ_CP118153:c1870843-1870436 [Pseudomonas chlororaphis]
ATGCTTTACCCGATTGCAATTTCAATGGGCGATGACAAACACGCCTGGGGGGTGGAAGTCCCCGACATTCCTGGCTGTTT
CTCGGCCGGTGACGACCTCGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATTCTCGCCGAAG
ACGGTGCCCCCATCCCGCACGCCAACAAGGTCACCCTGCACGCAGCCAACCCTCAGTACGCAGGCTGCACCTGGGCGCTG
ATCGACATCGATATCACCAAGTACCTGGGCAAGGCGCAGAAGCTCAACATCACCCTGCCCGGCTACTTGCTGAACCGCAT
CGACGAATACGTGCTGCATCACCCGGAAGCCAAGAGCCGCTCGGGGTTTCTCGCTTCGGCTGCACTGAAGGTTTTGCAGC
AAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References