Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5148502..5149127 Replicon chromosome
Accession NZ_CP086285
Organism Klebsiella pneumoniae strain Kp1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LL004_RS25220 Protein ID WP_002882817.1
Coordinates 5148502..5148885 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LL004_RS25225 Protein ID WP_004150355.1
Coordinates 5148885..5149127 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL004_RS25205 5145868..5146770 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LL004_RS25210 5146767..5147402 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LL004_RS25215 5147399..5148328 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LL004_RS25220 5148502..5148885 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LL004_RS25225 5148885..5149127 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LL004_RS25230 5149332..5150249 + 918 WP_110230358.1 alpha/beta hydrolase -
LL004_RS25235 5150263..5151204 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LL004_RS25240 5151249..5151686 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LL004_RS25245 5151683..5152543 - 861 WP_004146232.1 virulence factor BrkB family protein -
LL004_RS25250 5152537..5153136 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T222900 WP_002882817.1 NZ_CP086285:c5148885-5148502 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T222900 NZ_CP118152:c2685829-2685647 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAGCTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCAGTCAAAGGCAGTCATCACCAGTA
CAAACATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT222900 WP_004150355.1 NZ_CP086285:c5149127-5148885 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT222900 NZ_CP118152:c2685617-2685216 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCACTACGAAGGCCTGGTCGCCGATG
GTGAGCCGTTGCCCCAGGTGCATGAAGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGGGTGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCTGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGTTTTCTCGCGGCAGCGGCATTGCGCGAGTTGTCGGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References